Application | Comment | Organism |
---|---|---|
medicine | GSTs catalyzing inactivation of anticancer drugs may find clinical use in protecting sensitive normal tissues to toxic side effects in treated patients, and as selectable markers in gene therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of the His6-tagged isozyme GST T1-1 in Escherichia coli | Mus musculus |
recombinant expression of the His6-tagged wild-type and mutant isozymes GST T1-1 in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C14S/T226I/W234R | construction of chimeric mutant F2:1215 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F2:1215 shows highly increased activity compared to the human enzyme and increased activity compared to the murine enzyme, overview | Homo sapiens |
additional information | construction of chimeric mutants F2:1215 and F5:1474 comprising sequences from the murine isozymes GST-T1-1, as well as from the isozymes GST-T1-1 and GST-T2 of Homo sapiens, the mutant F2:1215 and mutant F5:1474 show increased activity compared to the human and to the murine enzyme, overview | Mus musculus |
W234R | construction of chimeric mutant F5:1474 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F5:1474 shows increased activity compared to the human enzyme and to the murine enzyme, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of recombinant His6-tagged isozyme GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea | Mus musculus | |
additional information | - |
additional information | steady-state kinetics of recombinant His6-tagged wild-type and mutant isozymes GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea | Homo sapiens | |
0.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
0.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens | |
0.8 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
1 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 | ? | - |
? | |
additional information | Mus musculus | the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme GST T1-1 | - |
Mus musculus | - |
isozyme GST T1-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Mus musculus | ? | - |
? | |
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione | - |
Homo sapiens | ? | - |
? | |
1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione | - |
Mus musculus | ? | - |
? | |
1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione | - |
Homo sapiens | ? | - |
? | |
4-nitrophenethyl bromide + glutathione | - |
Mus musculus | ? | - |
? | |
4-nitrophenethyl bromide + glutathione | - |
Homo sapiens | ? | - |
? | |
additional information | the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 | Homo sapiens | ? | - |
? | |
additional information | the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GST T1-1 | - |
Mus musculus |
GST T1-1 | - |
Homo sapiens |
theta-class glutathione transferase | - |
Mus musculus |
theta-class glutathione transferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens | |
2.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
3.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
3.6 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Homo sapiens | |
2.9 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 | Mus musculus | |
6.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Mus musculus | |
6.3 | - |
1,3-Bis-(2-chloroethyl)-1-nitrosourea | pH 7.4, 37°C, recombinant mutant F2:1215 | Homo sapiens |