Literature summary for 2.5.1.18 extracted from

Jang, E.H.; Park, H.; Park, A.K.; Moon, J.H.; Chi, Y.M.; Ahn, I.Y.
Crystallization and preliminary X-ray crystallographic studies of the rho-class glutathione S-transferase from the antarctic clam Laternula elliptica (2008), Acta Crystallogr. Sect. F, 64, 1132-1134.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3)	Laternula elliptica

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with substrates glutathione or 1-chloro-2,4-dinitrobenzene, sitting-drop vapour-diffusion method, 0.004 ml of protein solution, containing 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 2 mM DTT, is mixed with 0.004 ml of reservoir solution containing 6% Tacsimate, pH 8.0, and 26% w/v PEG 3350, for cocrystallization, the protein solution is mixed with glutathione at a molar ratio of 1:1 and with 1-chloro-2,4-dinitrobenzene at a molar ratio of 1:5. The glutathionecomplex crystals from 7% Tacsimate, pH 8.0, 24% w/v PEG 3350 and the CDNB-complex crystals from 8.5% Tacsimate, pH 8.0, 25% w/v PEG 335. Equilibration over 0.5 ml reservoir solution, 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.2 A and 2.0 A resolution, respectively	Laternula elliptica

Crystallization (Comment)

Organism

enzyme in complex with substrates glutathione or 1-chloro-2,4-dinitrobenzene, sitting-drop vapour-diffusion method, 0.004 ml of protein solution, containing 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 2 mM DTT, is mixed with 0.004 ml of reservoir solution containing 6% Tacsimate, pH 8.0, and 26% w/v PEG 3350, for cocrystallization, the protein solution is mixed with glutathione at a molar ratio of 1:1 and with 1-chloro-2,4-dinitrobenzene at a molar ratio of 1:5. The glutathionecomplex crystals from 7% Tacsimate, pH 8.0, 24% w/v PEG 3350 and the CDNB-complex crystals from 8.5% Tacsimate, pH 8.0, 25% w/v PEG 335. Equilibration over 0.5 ml reservoir solution, 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.2 A and 2.0 A resolution, respectively

Laternula elliptica

Organism

Organism	UniProt	Comment	Textmining
Laternula elliptica	-	Antarctic soft-shelled clam	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 95% purity	Laternula elliptica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Laternula elliptica	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	a rho-class glutathione S-transferase	Laternula elliptica
GST	-	Laternula elliptica
rho-GST	-	Laternula elliptica