Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Paragonimus westermani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25100 | - |
estimated from amino acid sequence | Paragonimus westermani |
30000 | - |
His-tagged recombinant enzyme, SDS-PAGE | Paragonimus westermani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paragonimus westermani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Paragonimus westermani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
using trans-4-phenyl-3-butene-2-one as a substrate | Paragonimus westermani |
325 | - |
using 1-chloro-2,4-dinitrobenzene as a substrate | Paragonimus westermani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Paragonimus westermani | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + trans-4-phenyl-3-butene-2-one | - |
Paragonimus westermani | ? | - |
? | |
additional information | no activity towards bromosulphophthalein, 1,2-dichloro-4-nitrobenzene, ethacrynic acid, 1,2-epoxy-2(p-nitrophenoxy) propane, trans-non-2-enal, and hexa-2,4-dienal | Paragonimus westermani | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
26GST | - |
Paragonimus westermani |
class mu glutathione S-transferase | - |
Paragonimus westermani |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paragonimus westermani | estimated from amino acid sequence | - |
5.7 |