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Literature summary for 2.5.1.18 extracted from
- Purification and biochemical characterization of cytosolic glutathione-S-transferase from filarial worms Setaria cervi (2008), Comp. Biochem. Physiol. B, 151, 237-245.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | a decrease in the activity of the enzyme is observed when glutathione concentration is increased beyond 1.0 mM | Setaria cervi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
glutathione | native enzyme | Setaria cervi | |
| 2.5 | - |
1-chloro-2,4-dinitrobenzene | native enzyme | Setaria cervi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Setaria cervi | 5829 | - |
| microsome | - |
Setaria cervi | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24600 | - |
2 * 24600, MALDI-TOF spectrometry | Setaria cervi |
| 49200 | - |
gel filtration | Setaria cervi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Setaria cervi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, carboxymethyl-Sepharose column chromatography, and GSH-Sepharose affinity chromatography followed by ultrafiltration | Setaria cervi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.041 | - |
cytosolic extract, using 1-chloro-2,4-dinitrobenzene as a substrate | Setaria cervi |
| 13.4 | - |
after 43.2fold purification, using 1-chloro-2,4-dinitrobenzene as a substrate | Setaria cervi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Setaria cervi | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| glutathione + cumene hydroperoxide | - |
Setaria cervi | ? | - |
? | |
| additional information | no activity with bromosulphathalein, 1,2-dichloro-4-nitrobenzene, t-butyl hydroperoxide, and linoleic hydroperoxide | Setaria cervi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 24600, MALDI-TOF spectrometry | Setaria cervi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Setaria cervi |
| GST | - |
Setaria cervi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | - |
Setaria cervi |
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