Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Anopheles dirus |
Protein Variants | Comment | Organism |
---|---|---|
F212A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F212E | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F212H | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F212L | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F212W | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F212Y | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
F216A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Q112A | slightly decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
R214A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
T174A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Y215A | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Y215E | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Y215F | increased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Y215H | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
Y215S | decreased activity towards 1-chloro-2,4-dinitrobenzene compared to the wild type enzyme | Anopheles dirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Ethacrynic acid | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.032 | - |
Ethacrynic acid | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.037 | - |
Ethacrynic acid | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.065 | - |
Ethacrynic acid | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.1 | - |
Ethacrynic acid | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.33 | - |
glutathione | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.35 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.38 | - |
glutathione | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.44 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.45 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.48 | - |
glutathione | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.52 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.53 | - |
glutathione | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.53 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.6 | - |
glutathione | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.62 | - |
glutathione | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.64 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.65 | - |
glutathione | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.67 | - |
glutathione | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.7 | - |
glutathione | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.73 | - |
glutathione | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.76 | - |
glutathione | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.78 | - |
glutathione | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.85 | - |
glutathione | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.85 | - |
glutathione | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.88 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.93 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.99 | - |
glutathione | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
1.01 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
1.77 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
1.78 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
1.92 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.03 | - |
glutathione | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.35 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.37 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
3.06 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
3.69 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles dirus | Q9GN60 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap affinity column chromatography | Anopheles dirus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
mutant enzyme F212H, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.005 | - |
mutant enzyme Y215E, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.006 | - |
mutant enzyme T174A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.006 | - |
mutant enzyme Y215A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.009 | - |
mutant enzyme F212Y, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.01 | - |
mutant enzyme F212H, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.012 | - |
mutant enzyme F212Y, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.017 | - |
mutant enzyme F212W, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.017 | - |
mutant enzyme R214A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.018 | - |
mutant enzyme Q112A, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.019 | - |
mutant enzyme Y215H, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.021 | - |
mutant enzyme F212W, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.025 | - |
mutant enzyme Y215A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.026 | - |
mutant enzyme Y215S, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.029 | - |
mutant enzyme Y215E, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.029 | - |
mutant enzyme Y215S, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.03 | - |
mutant enzyme Y215A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.03 | - |
wild type enzyme, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.034 | - |
mutant enzyme F212A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.035 | - |
mutant enzyme Y215E, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.036 | - |
mutant enzyme Q112A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.036 | - |
mutant enzyme Y215A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.04 | - |
mutant enzyme Y215H, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.041 | - |
mutant enzyme F212A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.043 | - |
mutant enzyme Y215F, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.044 | - |
mutant enzyme Q112A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.048 | - |
mutant enzyme R214A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.049 | - |
mutant enzyme Y215A, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.052 | - |
mutant enzyme R214A, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.053 | - |
mutant enzyme Y215H, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.059 | - |
mutant enzyme F212L, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.068 | - |
wild type enzyme, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.069 | - |
mutant enzyme Y215F, using 4-nitrophenethyl bromide as a substrate | Anopheles dirus |
0.073 | - |
mutant enzyme T174A, using ethacrynic acid as a substrate | Anopheles dirus |
0.079 | - |
wild type enzyme, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.085 | - |
mutant enzyme F212A, using ethacrynic acid as a substrate | Anopheles dirus |
0.086 | - |
mutant enzyme F212E, using ethacrynic acid as a substrate | Anopheles dirus |
0.086 | - |
mutant enzyme Y215F, using 4-nitrobenzyl chloride as a substrate | Anopheles dirus |
0.087 | - |
mutant enzyme F212L, using 1,2-dichloro-4-nitrobenzene as a substrate | Anopheles dirus |
0.12 | - |
mutant enzyme F212H, using ethacrynic acid as a substrate | Anopheles dirus |
0.17 | - |
mutant enzyme Y215H, using ethacrynic acid as a substrate | Anopheles dirus |
0.29 | - |
mutant enzyme Y215F, using ethacrynic acid as a substrate | Anopheles dirus |
0.3 | - |
mutant enzyme Y215E, using ethacrynic acid as a substrate | Anopheles dirus |
0.31 | - |
mutant enzyme F212Y, using ethacrynic acid as a substrate | Anopheles dirus |
0.34 | - |
mutant enzyme F212L, using ethacrynic acid as a substrate | Anopheles dirus |
0.37 | - |
mutant enzyme F212W, using ethacrynic acid as a substrate | Anopheles dirus |
0.47 | - |
mutant enzyme R214A, using ethacrynic acid as a substrate | Anopheles dirus |
0.47 | - |
mutant enzyme Y215A, using ethacrynic acid as a substrate | Anopheles dirus |
0.51 | - |
mutant enzyme F212E, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
0.57 | - |
wild type enzyme, using ethacrynic acid as a substrate | Anopheles dirus |
0.61 | - |
mutant enzyme Q112A, using ethacrynic acid as a substrate | Anopheles dirus |
0.64 | - |
mutant enzyme Y215S, using ethacrynic acid as a substrate | Anopheles dirus |
1.03 | - |
mutant enzyme Y215A, using ethacrynic acid as a substrate | Anopheles dirus |
3.03 | - |
mutant enzyme T174A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
4.28 | - |
mutant enzyme F212H, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
8.03 | - |
mutant enzyme F212Y, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
12.2 | - |
mutant enzyme F212A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
12.3 | - |
mutant enzyme F212W, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
22.9 | - |
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
25.2 | - |
mutant enzyme Y215A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
26.1 | - |
mutant enzyme Y215E, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
27 | - |
mutant enzyme Y215S, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
32.2 | - |
mutant enzyme F212L, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
36.2 | - |
mutant enzyme Y215H, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
44.3 | - |
mutant enzyme R214A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
52.4 | - |
mutant enzyme Q112A, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
58.6 | - |
wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
74.3 | - |
mutant enzyme Y215F, using 1-chloro-2,4-dinitrobenzene as a substrate | Anopheles dirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-dichloro-4-nitrobenzene | weak substrate | Anopheles dirus | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | best substrate | Anopheles dirus | S-(2,4-dinitrophenyl)-glutathione + HCl | - |
? | |
glutathione + 4-nitrobenzyl chloride | weak substrate | Anopheles dirus | ? | - |
? | |
glutathione + 4-nitrophenethyl bromide | weak substrate | Anopheles dirus | ? | - |
? | |
glutathione + ethacrynic acid | weak substrate | Anopheles dirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GSTD4-4 | - |
Anopheles dirus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the wild type enzyme shows a half-life of 13.3 min at 45°C | Anopheles dirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Ethacrynic acid | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.28 | - |
Ethacrynic acid | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.36 | - |
Ethacrynic acid | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.38 | - |
Ethacrynic acid | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.4 | - |
Ethacrynic acid | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
0.54 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
2.14 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
3.55 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
6.44 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
8.85 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
12.6 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
12.8 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
13.9 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
14.9 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
17 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
18.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
20.5 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
20.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
26.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
26.5 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus | |
35.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5 | Anopheles dirus |