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Literature summary for 2.5.1.18 extracted from
- Glutathione transferase activity with a novel substrate mimics the activation of the prodrug azathioprine (2008), Anal. Biochem., 375, 339-344.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
| expressed in Escherichia coli | Bos taurus |
| expressed in Escherichia coli | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Homo sapiens | P08263 | - |
- |
| Homo sapiens | Q16772 | - |
- |
| Rattus norvegicus | P04903 | - |
- |
| Rattus norvegicus | P04904 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-immobilized metal ion affinity chromatography | Homo sapiens |
| nickel-immobilized metal ion affinity chromatography | Bos taurus |
| nickel-immobilized metal ion affinity chromatography | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
isozyme GST A1-1, using azathioprine as a substrate | Bos taurus |
| 0.005 | - |
isozyme GST A2-2, using azathioprine as a substrate | Rattus norvegicus |
| 0.01 | - |
isozyme GST A3-3, using azathioprine as a substrate | Homo sapiens |
| 0.023 | - |
isozyme GST A3-3, using azathioprine as a substrate | Rattus norvegicus |
| 0.035 | - |
isozyme GST A3-3, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Rattus norvegicus |
| 0.21 | - |
isozyme GST A1-1, using azathioprine as a substrate | Homo sapiens |
| 0.53 | - |
isozyme GST A2-2, using azathioprine as a substrate | Homo sapiens |
| 2.57 | - |
isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Rattus norvegicus |
| 4.58 | - |
isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Bos taurus |
| 7.26 | - |
isozyme GST A3-3, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Homo sapiens |
| 53.2 | - |
isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Homo sapiens |
| 61.8 | - |
isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole | - |
Homo sapiens | ? | - |
? |  |
| glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole | - |
Bos taurus | ? | - |
? | |
| glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole | - |
Rattus norvegicus | ? | - |
? | |
| glutathione + azathioprine | - |
Homo sapiens | 6-mercaptopurine + ? | - |
? | |
| glutathione + azathioprine | - |
Bos taurus | 6-mercaptopurine + ? | - |
? | |
| glutathione + azathioprine | - |
Rattus norvegicus | 6-mercaptopurine + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Homo sapiens |
| glutathione S-transferase | - |
Bos taurus |
| glutathione S-transferase | - |
Rattus norvegicus |
| GST A1-1 | isozyme | Bos taurus |
| GST A1-1 | isozyme | Homo sapiens |
| GST A2-2 | isozyme | Homo sapiens |
| GST A2-2 | isozyme | Rattus norvegicus |
| GST A3-3 | isozyme | Homo sapiens |
| GST A3-3 | isozyme | Rattus norvegicus |
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Release 2023.1 (January 2023)
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