Application | Comment | Organism |
---|---|---|
medicine | isozyme GST-1 is a vaccine target | Ancylostoma caninum |
Cloned (Comment) | Organism |
---|---|
gene gst-1, DNA and amino acid sequence determination and analysis, expression of His-tagged GST-1 in Pichia pastoris strain X-33 | Ancylostoma caninum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, isozyme GST-1, SDS-PAGE | Ancylostoma caninum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + hematin | Ancylostoma caninum | GST-1 is involved in parasite heme detoxification during hemoglobin digestion or heme uptake | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ancylostoma caninum | Q6J1M5 | GST-1; canine hookworm, gene gst-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged GST-1 from Pichia pastoris strain X-33 by nickel affinity chromatography | Ancylostoma caninum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | isozyme GST-1 | Ancylostoma caninum | - |
hypodermis | - |
Ancylostoma caninum | - |
intestine | low activity | Ancylostoma caninum | - |
additional information | developmental expression profile, mRNA encoding GST-1 is detected in adults, eggs, and larval stages, but the protein is detected only in adult hookworm somatic extracts and excretory/secretory products, overview | Ancylostoma caninum | - |
muscle | - |
Ancylostoma caninum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Ancylostoma caninum | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + hematin | GST-1 is involved in parasite heme detoxification during hemoglobin digestion or heme uptake | Ancylostoma caninum | ? | - |
? | |
glutathione + hematin | high affinity binding by GST-1 | Ancylostoma caninum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, isozyme GST-1, SDS-PAGE | Ancylostoma caninum |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Ancylostoma caninum |
GST-1 | - |
Ancylostoma caninum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ancylostoma caninum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Ancylostoma caninum |