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Literature summary for 2.5.1.18 extracted from

  • Wongtrakul, J.; Sramala, I.; Prapanthadara, L.A.; Ketterman, A.J.
    Intra-subunit residue interactions from the protein surface to the active site of glutathione S-transferase AdGSTD3-3 impact on structure and enzyme properties (2005), Insect Biochem. Mol. Biol., 35, 197-205.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D150A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.3fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value Anopheles dirus
D150S turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.1fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value Anopheles dirus
D150Y turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.7fold higher than wild-type value Anopheles dirus
H144A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is nearly identical to wild-type value Anopheles dirus
R96A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.9fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold higher than wild-type value Anopheles dirus
V147A turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene nearly identical to wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold lower than wild-type value Anopheles dirus
V147L turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold lower than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.8fold lower than wild-type value Anopheles dirus

Inhibitors

Inhibitors Comment Organism Structure
1,2-dichloro-4-nitrobenzene 1 mM, 53.3% inhibition of wild-type enzyme, 40.4% of mutant enzyme H144A, 42.4% of mutant enzyme V147A, 54.3% of mutant enzyme V147L, 35.9% of mutant enzyme R96A Anopheles dirus
4-nitrobenzyl chloride 1 mM, 50.5% inhibition of wild-type enzyme, 43.9% of mutant enzyme H144A, 40.8% of mutant enzyme V147A, 47.9% of mutant enzyme V147L, 30.8% of mutant enzyme R96A Anopheles dirus
4-nitrophenethyl bromide 0.1 mM, 40.1% inhibition of wild-type enzyme, 23.6% of mutant enzyme H144A, 19.5% of mutant enzyme V147A, 31.4% of mutant enzyme V147L, 27.3% of mutant enzyme R96A Anopheles dirus
cumene hydroperoxide 2.5 mM, 43.3% inhibition of wild-type enzyme, 27% of mutant enzyme H144A, 50.6% of mutant enzyme V147A, 41.5% of mutant enzyme V147L, 48.1% of mutant enzyme R96A Anopheles dirus
deltamethrin 0.01 mM, 65.4% inhibition of wild-type enzyme, 44.2% of mutant enzyme H144A, 59.5% of mutant enzyme V147A, 66.1% of mutant enzyme V147L, 88.6% of mutant enzyme R96A Anopheles dirus
Ethacrynic acid 0.001 mM, 35.3% inhibition of wild-type enzyme, 32.7% of mutant enzyme H144A, 27.8% of mutant enzyme V147A, 23.0% of mutant enzyme V147L, 29.1% of mutant enzyme R96A Anopheles dirus
permethrin 0.01 mM, 51.1% inhibition of wild-type enzyme, 38.8% of mutant enzyme H144A, 53.1% of mutant enzyme V147A, 49% of mutant enzyme V147L, 65.5% of mutant enzyme R96A Anopheles dirus
S-hexyl glutathione 0.01 mM, 21.7% inhibition of wild-type enzyme, 23% of mutant enzyme H144A, 10.8% of mutant enzyme V147A, 24.2% of mutant enzyme V147L, 14.7% of mutant enzyme R96A Anopheles dirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
0.08
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
0.09
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
0.13
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
0.14
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
0.17
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
0.19
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
0.2
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
0.21
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
0.26
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
0.29
-
reduced glutathione 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
0.33
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
0.34
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
0.37
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
0.38
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus
2.75
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus

Organism

Organism UniProt Comment Textmining
Anopheles dirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced glutathione + 1-chloro-2,4-dinitrobenzene
-
Anopheles dirus S-2,4-dinitrophenylglutathione + HCl
-
?
reduced glutathione + 4-nitrobenzyl chloride
-
Anopheles dirus ?
-
?
reduced glutathione + 4-nitrophenethyl bromide
-
Anopheles dirus ?
-
?
reduced glutathione + ethacrynic acid
-
Anopheles dirus ?
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase AdFSTD3-3
-
Anopheles dirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.6
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
21.6
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147L Anopheles dirus
24.9
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
24.9
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme V147A Anopheles dirus
25.2
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
25.2
-
reduced glutathione 25-27°C, pH 6.5, wild-type enzyme Anopheles dirus
27.8
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
27.8
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150S Anopheles dirus
29.1
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
29.1
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme H144A Anopheles dirus
29.5
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
29.5
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150Y Anopheles dirus
31.7
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
31.7
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme D150A Anopheles dirus
46.8
-
1-chloro-2,4-dinitrobenzene 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus
46.8
-
reduced glutathione 25-27°C, pH 6.5, mutant enzyme R96A Anopheles dirus