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Literature summary for 2.5.1.18 extracted from

  • Asaoka, K.; Takahashi, K.
    Purification and properties of porcine brain glutathione S-transferases (1983), J. Biochem., 94, 1191-1199.
    View publication on PubMed

General Stability

General Stability Organism
loss of activity during dialysis Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-
microsome
-
Sus scrofa
-
-
mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
2 * 22000, SDS-PAGE Sus scrofa
43000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity, 4 enzyme fractions Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain distribution in brain Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
124
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dinitrobenzene
-
Sus scrofa ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Sus scrofa S-2,4-dinitrophenylglutathione + HCl
-
?
glutathione + 2,5-dinitrophenol
-
Sus scrofa ?
-
?
glutathione + 3,4-dinitrobenzoic acid
-
Sus scrofa ?
-
?
glutathione + 4-nitrobenzyl chloride
-
Sus scrofa ?
-
?
glutathione + 5-nitrofurfural
-
Sus scrofa ?
-
?
glutathione + 5-nitrofurfural diacetal
-
Sus scrofa ?
-
?
additional information specificity Sus scrofa ?
-
?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Sus scrofa HX + R-S-glutathione
-
?

Subunits

Subunits Comment Organism
dimer 2 * 22000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 8.1 at pH 6.0 and 8.1 about 50% of activity maximum Sus scrofa