Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium fredii | - |
HH103, strain SVQ336 | - |
Sinorhizobium fredii | - |
HH103, strain SVQ524 | - |
Synonyms | Comment | Organism |
---|---|---|
cobO mutant SVQ336 | - |
Sinorhizobium fredii |
cobO mutant SVQ524 | - |
Sinorhizobium fredii |
cobO mutantSVQ336 | - |
Sinorhizobium fredii |
General Information | Comment | Organism |
---|---|---|
malfunction | a HH103 cobO mutant (strain SVQ524), is constructed by the insertion of omega interposon in the BglII site of cobO gene. Mutant is auxotroph for methionine and cobalamin as it is shown that the presence of either compound restores the growth on minimal medium. Mutant SVQ524 fails to nodulate on Vigna radiate but is able to nodulate on Glycine max cvs. Williams and Peking and Cajanus cajan. The roots of mutant plants do not secrete enough cobalamin and/or methionine to support growth of cobalamin/methionine auxotrophs in the rhizosphere. The phenotype of SVQ524 is rescued by the addition of methionine or cobalamin to the plant growth media or by the presence of a copy of the cobO gene | Sinorhizobium fredii |
malfunction | a methionine and cobalamin mutant strain (SVQ336) of Sinorhizobium fredii HH103 is obtained by Tn5-lacZ mutagenesis. Sequence analysis show that the transposon is inserted into a gene homologous to cobO. cobO mutant SVQ336 is auxotroph for methionine and cobalamin as it is shown that the presence of either compound restores the growth of SVQ336 on minimal medium | Sinorhizobium fredii |