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Literature summary for 2.5.1.17 extracted from

  • Johnson, C.L.; Pechonick, E.; Park, S.D.; Havemann, G.D.; Leal, N.A.; Bobik, T.A.
    Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene (2001), J. Bacteriol., 183, 1577-1584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.17

Cloned(Commentary)

Cloned (Comment) Organism
structural gene cobA Salmonella enterica
structural gene pduO Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Salmonella enterica
K+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
-
 Salmonella enterica
Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36600
-
 calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
37000
-
 SDS-PAGE of recombinant enzyme expressed in E. coli Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Salmonella enterica subsp. enterica serovar Typhimurium the main role of this enzyme is apparently the conversion of inactive cobalamins to adenosyl cobalamin for 1,2 propanediol degradation triphosphate + coenzyme B12
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 serovar typhimurium
-
Salmonella enterica subsp. enterica serovar Typhimurium P31570 serovar typhimurium, structural gene pduO, this DNA sequence encodes for several enzymes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.312
-
 pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.312
-
 activity of inclusion bodies formed with enzyme expressed in E.coli Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin
-
 Salmonella enterica triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin
-
 Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin the main role of this enzyme is apparently the conversion of inactive cobalamins to adenosyl cobalamin for 1,2 propanediol degradation Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + coenzyme B12
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium