BRENDA - Enzyme Database
show all sequences of 2.5.1.17

Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans

Debussche, L.; Couder, M.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 173, 6300-6302 (1991)

Data extracted from this reference:

General Stability
General Stability
Organism
DTT stabilizes during purification and storage, removal leads to complete loss of activity
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors
Inhibitors
Commentary
Organism
Structure
hydrogenobyrinic acid a,c-diamide
cobalt-free analogue of cobyrinic acid a,c-diamide
Pseudomonas denitrificans (nomen rejiciendum)
additional information
no inhibition by cobyrinic acid, hydrogenobyrinic acid
Pseudomonas denitrificans (nomen rejiciendum)
tripolyphosphate
-
Pseudomonas denitrificans (nomen rejiciendum)
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Pseudomonas denitrificans (nomen rejiciendum)
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
-
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
56000
-
HPLC gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
70000
-
PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + cob(I)alamin
Pseudomonas denitrificans (nomen rejiciendum)
-
triphosphate + coenzyme B12
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
P29930
recombinant strain SC510 Rifr containing plasmid pXL227, this DNA seuqence encodes for several enzymes
-
Purification (Commentary)
Commentary
Organism
centrifugation, Mono Q chromatography and gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.089
-
in the presence of cobalamin as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.13
-
in the presence of cobyrinic acid a,c-diamide as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.14
-
in the presence of cobyric acid as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.18
-
in the presence of cobinamide as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
-
-
-
-
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(I)inamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yric acid + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
additional information
cobyrinic acid is not a substrate
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
General Stability (protein specific)
General Stability
Organism
DTT stabilizes during purification and storage, removal leads to complete loss of activity
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydrogenobyrinic acid a,c-diamide
cobalt-free analogue of cobyrinic acid a,c-diamide
Pseudomonas denitrificans (nomen rejiciendum)
additional information
no inhibition by cobyrinic acid, hydrogenobyrinic acid
Pseudomonas denitrificans (nomen rejiciendum)
tripolyphosphate
-
Pseudomonas denitrificans (nomen rejiciendum)
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Pseudomonas denitrificans (nomen rejiciendum)
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
-
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
56000
-
HPLC gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
70000
-
PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + cob(I)alamin
Pseudomonas denitrificans (nomen rejiciendum)
-
triphosphate + coenzyme B12
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
centrifugation, Mono Q chromatography and gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.089
-
in the presence of cobalamin as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.13
-
in the presence of cobyrinic acid a,c-diamide as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.14
-
in the presence of cobyric acid as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.18
-
in the presence of cobinamide as substrate; pH 8.0, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
-
-
-
-
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
cob(I)inamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yric acid + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
cob(I)yrinic acid a,c-diamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
additional information
cobyrinic acid is not a substrate
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 2.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738459
Pallares
Spectroscopic Studies of the E ...
Salmonella enterica
J. Am. Chem. Soc.
138
3694-3704
2016
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737450
Park
Unprecedented mechanism employ ...
Salmonella enterica
Angew. Chem. Int. Ed. Engl.
54
7158-7161
2015
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737702
Pallares
Spectroscopic studies of the S ...
Salmonella enterica
Biochemistry
53
7969-7982
2014
-
-
1
-
6
-
-
2
-
-
-
2
-
3
-
-
1
-
-
-
-
-
5
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
5
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
738536
Moore
The EutT enzyme of Salmonella ...
Salmonella enterica, Salmonella enterica JE6583
J. Bacteriol.
196
903-910
2014
1
-
1
-
4
-
-
1
-
1
1
-
-
3
1
-
1
-
-
-
-
1
6
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
1
-
1
1
-
-
1
-
1
-
-
-
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
721682
Moore
Structural insights into the m ...
Salmonella enterica
Biochemistry
51
9647-9657
2012
-
-
1
1
12
-
-
18
-
1
-
1
-
2
-
-
1
2
-
-
-
-
1
-
1
-
-
14
1
-
-
-
-
-
-
-
-
1
-
1
12
-
-
-
-
18
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
14
1
-
-
-
-
3
3
-
18
18
722386
Park
Spectroscopic characterization ...
Lactobacillus reuteri
Inorg. Chem.
51
4482-4494
2012
-
-
1
-
8
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721492
Park
Crystal structure of PduO-Type ...
Bacillus cereus
Biochem. Biophys. Res. Commun.
408
417-421
2011
-
-
1
1
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
704686
Mera
Dihydroflavin-driven adenosyla ...
Homo sapiens, Lactobacillus reuteri
J. Biol. Chem.
285
2911-2917
2010
-
-
2
-
1
-
-
-
-
-
-
2
-
2
-
-
2
-
-
-
8
-
3
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
8
-
3
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
701946
Medina
Mutation in the cobO gene gene ...
Sinorhizobium fredii
Arch. Microbiol.
191
11-21
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
702286
Mera
Residue Phe112 of the human-ty ...
Lactobacillus reuteri
Biochemistry
48
3138-3145
2009
-
-
1
-
7
-
-
29
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
29
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
29
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
29
1
-
-
-
-
-
-
-
29
29
702311
Padovani
A rotary mechanism for coenzym ...
Methylorubrum extorquens
Biochemistry
48
5350-5357
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
705758
Zhang
Ligand-binding by catalyticall ...
Homo sapiens
Mol. Genet. Metab.
98
278-284
2009
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
690265
Park
Crystallization and preliminar ...
Bacillus cereus
Acta Crystallogr. Sect. F
64
648-650
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690933
Fan
Functional characterization an ...
Homo sapiens
Biochemistry
47
2806-2813
2008
-
1
1
-
32
-
-
32
-
-
-
-
-
2
-
-
1
-
-
-
1
-
4
-
-
-
-
33
-
-
-
-
-
-
-
-
1
1
-
-
32
-
-
-
-
32
-
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
33
-
-
-
-
-
-
-
-
-
-
690964
St Maurice
Structural characterization of ...
Lactobacillus reuteri
Biochemistry
47
5755-5766
2008
-
1
-
1
-
-
1
-
-
-
-
-
-
3
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
695036
Moon
Crystal structure of a PduO-ty ...
Burkholderia thailandensis
Proteins
72
1066-1070
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702245
Park
Kinetic and spectroscopic stud ...
Lactobacillus reuteri
Biochemistry
47
9007-9015
2008
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
674853
St.Maurice
Structural characterization of ...
Lactobacillus reuteri, Lactobacillus reuteri CRL1098
J. Biol. Chem.
282
2596-2605
2007
-
-
1
1
-
-
-
2
-
1
-
2
-
5
-
-
1
2
-
-
-
-
4
-
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
2
-
1
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
675180
Erger
In vivo expression of human AT ...
Homo sapiens
J. Gene Med.
9
462-469
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682763
Tanaka
Molecular properties of two pr ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Proteins
68
446-457
2007
-
-
1
1
-
3
-
-
1
-
7
-
1
6
-
-
1
1
-
-
-
-
6
2
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
3
-
-
-
-
1
-
8
-
1
-
-
2
-
-
-
-
6
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690887
Mera
Structural and functional anal ...
Lactobacillus reuteri
Biochemistry
46
13829-13836
2007
-
-
1
1
7
-
2
7
-
-
-
-
-
5
-
-
-
1
-
-
-
-
7
1
-
-
-
29
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
2
-
7
-
-
-
-
-
-
-
-
-
-
-
-
7
1
-
-
-
29
-
-
-
-
-
-
-
-
-
-
672110
Schubert
Structure of ATP-bound human A ...
Homo sapiens
Biochemistry
45
15188-15196
2006
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674277
Buan
Studies of the CobA-type ATP:C ...
Methanosarcina mazei
J. Bacteriol.
188
3543-3550
2006
-
-
1
-
1
-
2
2
1
5
-
2
-
6
-
-
1
-
-
-
1
-
5
-
1
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
1
-
-
2
-
2
1
5
-
2
-
-
-
1
-
-
1
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
674576
Buan
Purification and initial bioch ...
Salmonella enterica
J. Biol. Chem.
281
16971-16977
2006
-
-
1
-
3
-
5
3
-
2
-
8
-
3
-
-
1
2
1
-
1
-
9
-
1
-
-
2
1
1
-
8
-
-
-
-
-
1
8
-
3
-
-
5
-
3
-
2
-
8
-
-
-
1
1
-
1
-
9
-
1
-
-
2
1
1
-
-
-
-
-
-
-
-
676023
Zhang
Impact of cblB mutations on th ...
Homo sapiens
Mol. Genet. Metab.
87
315-322
2006
-
-
1
-
2
-
-
5
1
1
-
2
-
2
-
-
1
-
-
2
2
-
3
-
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
5
1
1
-
2
-
-
-
1
-
2
2
-
3
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
658991
Stich
Spectroscopic evidence for the ...
Homo sapiens
J. Am. Chem. Soc.
127
7660-7661
2005
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658993
Stich
Spectroscopic and computationa ...
Salmonella enterica
J. Am. Chem. Soc.
127
8710-8719
2005
-
-
-
-
-
1
-
-
-
-
-
2
-
3
1
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
674510
Buan
Computer-assisted docking of f ...
Salmonella enterica
J. Biol. Chem.
280
40948-40956
2005
-
-
1
-
12
-
1
1
-
1
-
1
-
4
-
-
1
2
-
-
2
-
2
-
1
-
-
-
-
-
-
2
1
-
-
-
-
1
2
-
12
-
-
1
1
1
-
1
-
1
-
-
-
1
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
652541
Saridakis
The structural basis for methy ...
Thermoplasma acidophilum
J. Biol. Chem.
279
23646-23653
2004
1
-
1
1
8
2
-
9
-
4
-
2
-
3
-
-
-
-
-
-
6
-
7
2
1
1
-
6
1
-
-
-
-
-
-
1
-
1
-
1
8
2
-
-
-
9
-
4
-
2
-
-
-
-
-
-
6
-
7
2
1
1
-
6
1
-
-
-
-
-
-
-
-
-
659048
Buan
The eutT gene of Salmonella en ...
Salmonella enterica, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
186
5708-5714
2004
2
-
2
-
-
-
1
-
-
1
-
4
-
4
2
-
-
-
-
-
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
1
-
4
-
2
-
-
-
-
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
659058
Johnson
Purification and initial chara ...
Salmonella enterica
J. Bacteriol.
186
7881-7887
2004
-
-
1
-
1
-
9
3
-
5
1
1
-
3
-
-
1
-
1
-
3
-
4
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
9
-
3
-
5
1
1
-
-
-
1
1
-
3
-
4
2
1
-
-
1
1
-
-
-
-
-
-
-
-
-
659411
Leal
Human ATP:cob(I)alamin adenosy ...
Homo sapiens
J. Biol. Chem.
279
47536-47542
2004
-
-
1
-
-
-
-
5
1
1
-
2
-
3
-
-
1
-
-
-
2
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
1
1
-
2
-
-
-
1
-
-
2
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636875
Leal
Identification of the human an ...
Bos taurus, Homo sapiens
J. Biol. Chem.
278
9227-9234
2003
-
-
2
-
-
-
-
-
1
4
6
-
-
6
-
-
1
-
-
3
4
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
4
6
-
-
-
-
1
-
3
4
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
636874
Fonseca
The ATP:co(I)rrinoid adenosylt ...
Salmonella enterica
J. Biol. Chem.
277
33127-33131
2002
-
-
-
-
-
-
3
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636872
Fonseca
An in vitro reducing system fo ...
Salmonella enterica
J. Biol. Chem.
276
32101-32108
2001
-
-
-
-
-
-
-
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636873
Johnson
Functional genomic, biochemica ...
Salmonella enterica, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
183
1577-1584
2001
-
-
2
-
-
-
-
-
-
4
2
1
-
3
-
-
-
-
-
-
1
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
2
1
-
-
-
-
-
-
1
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
636871
Suh
Purification and initial chara ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
177
921-925
1995
-
-
-
-
-
1
-
2
1
4
2
-
-
2
-
-
1
-
-
-
1
-
1
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
4
2
-
-
-
-
1
-
-
1
-
1
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
636868
Debussche
Purification and partial chara ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6300-6302
1991
-
-
-
-
-
1
3
-
1
1
3
1
-
2
-
-
1
-
-
-
4
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
1
3
1
-
-
-
1
-
-
4
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636869
Crouzet
Nucleotide sequence and geneti ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6074-6087
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636867
Parry
-
Studies of enzyme stereochemis ...
Clostridium tetanomorphum
J. Am. Chem. Soc.
107
2190-2191
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636870
Sato
Assay, purification and charac ...
Serratia plymuthica
J. Nutr. Sci. Vitaminol.
30
405-413
1984
-
-
-
-
-
-
-
1
-
2
1
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636866
Beck
Ribosome-associated vitamin B1 ...
Clostridium tetanomorphum, Lactobacillus delbrueckii, Lactobacillus leichmannii, Propionibacterium freudenreichii subsp. shermanii
Methods Enzymol.
67
41-56
1980
-
-
-
-
-
4
8
1
4
10
-
4
-
4
-
-
1
-
-
-
1
4
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
1
4
10
-
4
-
-
-
1
-
-
1
4
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
636865
Mudd
-
The adenosyltransferases ...
Clostridium tetanomorphum, Propionibacterium freudenreichii subsp. shermanii
The Enzymes, 3rd. Ed. (Boyer P. D. ed. )
8
121-154
1973
-
-
-
-
-
-
4
2
-
10
-
2
-
2
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
10
-
2
-
-
-
1
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636864
Vitols
Enzymatic conversion of vitami ...
Clostridium tetanomorphum
J. Biol. Chem.
241
1455-1461
1966
-
-
-
-
-
2
5
2
1
5
-
1
-
1
-
-
1
-
-
-
-
3
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
2
1
5
-
1
-
-
-
1
-
-
-
3
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-