Crystallization (Comment) | Organism |
---|---|
native enzyme and complexes with inhibitor BPH-629, and mutants Y51F with TPP, mutant Y51F/Y90F with TPP, to 1.8-2.7 A resolution | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D34A | compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol | Mycobacterium tuberculosis |
R38A | compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol | Mycobacterium tuberculosis |
Y51F | production of both (13S)-isotuberculosinol and tuberculosinol is reduced | Mycobacterium tuberculosis |
Y51F/Y90F | no enzymic activity | Mycobacterium tuberculosis |
Y90F | compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJ61 | bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9 | - |
Mycobacterium tuberculosis ATCC 25618 | P9WJ61 | bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | tuberculosinol + diphosphate | reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
tuberculosinyl diphosphate + H2O | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | Mycobacterium tuberculosis | tuberculosinol + diphosphate | - |
? | |
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | tuberculosinol + diphosphate | reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
tuberculosinyl diphosphate + H2O | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | Mycobacterium tuberculosis ATCC 25618 | tuberculosinol + diphosphate | - |
? | |
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | (13S)-isotuberculosinol + diphosphate | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | (13S)-isotuberculosinol + diphosphate | reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | (13S)-isotuberculosinol + diphosphate | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | (13S)-isotuberculosinol + diphosphate | reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? |
Synonyms | Comment | Organism |
---|---|---|
Rv3378c | - |
Mycobacterium tuberculosis |