Literature summary for 2.5.1.153 extracted from

Hoshino, T.; Nakano, C.; Ootsuka, T.; Shinohara, Y.; Hara, T.
Substrate specificity of Rv3378c, an enzyme from Mycobacterium tuberculosis, and the inhibitory activity of the bicyclic diterpenoids against macrophage phagocytosis (2011), Org. Biomol. Chem., 9, 2156-2165.

Search for more literature for 2.5.1.153

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for the enzymatic activities	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycobacterium tuberculosis H37Rv	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 tuberculosinyl diphosphate + 2 H2O	-	Mycobacterium tuberculosis	(13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate	the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3	?
2 tuberculosinyl diphosphate + 2 H2O	-	Mycobacterium tuberculosis H37Rv	(13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate	the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3	?
copalyl diphosphate + H2O	-	Mycobacterium tuberculosis	(+)-copalol + manool + 13-epi-manool + diphosphate	40% (+)-copalol, 12% manool and 15% 13-epi-manool	?
copalyl diphosphate + H2O	-	Mycobacterium tuberculosis H37Rv	(+)-copalol + manool + 13-epi-manool + diphosphate	40% (+)-copalol, 12% manool and 15% 13-epi-manool	?
ent-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis	(-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate	22% (-)-ent copalol, 8% ent-13-epi-mancol, and 42% ent-manool	?
ent-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis H37Rv	(-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate	22% (-)-ent copalol, 8% ent-13-epi-mancol, and 42% ent-manool	?
additional information	no activity with terpentedienyl diphosphate, geranylgeranyl diphosphate, farnesyl diphosphate, and geranyl diphosphate	Mycobacterium tuberculosis	?	-	?
additional information	no activity with terpentedienyl diphosphate, geranylgeranyl diphosphate, farnesyl diphosphate, and geranyl diphosphate	Mycobacterium tuberculosis H37Rv	?	-	?
syn-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis	syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate	16% syn-copalol, 3% (13R)-vitrxifolin and 59% (13S)-vitexifolin	?
syn-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis H37Rv	syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate	16% syn-copalol, 3% (13R)-vitrxifolin and 59% (13S)-vitexifolin	?
tuberculosinyl diphosphate + H2O	-	Mycobacterium tuberculosis	tuberculosinol + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
Rv3378c protein	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)