Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta(DE3)pLysS | Plasmodium vivax |
Protein Variants | Comment | Organism |
---|---|---|
A383G | mutation does not much influence the enzyme catalytic activity. Mutant becomes 13fold more resistant to sulfadoxine than the wild type | Plasmodium vivax |
A383G/A553G | mutation does not much influence the enzyme catalytic activity. Mutant becomes 13fold more resistant to sulfadoxine than the wild type | Plasmodium vivax |
S382A/A383G/A553G | mutation does not much influence the enzyme catalytic activity. Mutant becomes 13fold more resistant to sulfadoxine than the wild type | Plasmodium vivax |
V585A | 2fold increase in the level of resistance to sulfadoxine compared with that of the wild type carrying V585A. V585A mutant is 4fold more resistant to dapsone than the wild type | Plasmodium vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dapsone | - |
Plasmodium vivax | |
sulfadoxine | - |
Plasmodium vivax | |
sulfathiazole | - |
Plasmodium vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
4-Aminobenzoate | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.00019 | - |
4-Aminobenzoate | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.00026 | - |
4-Aminobenzoate | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.00033 | - |
4-Aminobenzoate | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.00048 | - |
4-Aminobenzoate | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
174000 | - |
gel filtration | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate | Plasmodium vivax | - |
diphosphate + 7,8-dihydropteroate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Plasmodium vivax | diphosphate + 7,8-dihydropteroate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 85000, SDS-PAGE | Plasmodium vivax |
Synonyms | Comment | Organism |
---|---|---|
DHPS-HPPK | bifunctional enzyme | Plasmodium vivax |
dihydropteroate synthase/hydroxymethyldihydropterin pyrophosphokinase | bifunctional enzyme | Plasmodium vivax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
4-Aminobenzoate | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.0024 | - |
(7,8-dihydropterin-6-yl)methyl diphosphate | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.0029 | - |
4-Aminobenzoate | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.0029 | - |
(7,8-dihydropterin-6-yl)methyl diphosphate | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.0055 | - |
4-Aminobenzoate | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.0055 | - |
(7,8-dihydropterin-6-yl)methyl diphosphate | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.006 | - |
4-Aminobenzoate | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.006 | - |
(7,8-dihydropterin-6-yl)methyl diphosphate | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.0134 | - |
4-Aminobenzoate | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.0134 | - |
(7,8-dihydropterin-6-yl)methyl diphosphate | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
dapsone | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.00015 | - |
sulfathiazole | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.00019 | - |
sulfathiazole | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.00053 | - |
dapsone | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.0015 | - |
sulfadoxine | wild-type enzyme, pH 9.0, 37°C | Plasmodium vivax | |
0.00295 | - |
sulfathiazole | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.003 | - |
sulfadoxine | mutant enzyme V585A, pH 9.0, 37°C | Plasmodium vivax | |
0.00367 | - |
dapsone | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.00454 | - |
dapsone | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.01553 | - |
sulfathiazole | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.01773 | - |
dapsone | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.019 | - |
sulfathiazole | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.0481 | - |
sulfadoxine | mutant enzyme A383G, pH 9.0, 37°C | Plasmodium vivax | |
0.176 | - |
sulfadoxine | mutant enzyme A383G/A553G, pH 9.0, 37°C | Plasmodium vivax | |
0.227 | - |
sulfadoxine | mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C | Plasmodium vivax |
General Information | Comment | Organism |
---|---|---|
drug target | the enzyme is a sulfa drug target in malaria treatment | Plasmodium vivax |