Literature summary for 2.5.1.15 extracted from

Morgan, R.E.; Batot, G.O.; Dement, J.M.; Rao, V.A.; Eadsforth, T.C.; Hunter, W.N.
Crystal structures of Burkholderia cenocepacia dihydropteroate synthase in the apo-form and complexed with the product 7,8-dihydropteroate (2011), BMC Struct. Biol., 11, 21.

Application

Application	Comment	Organism
medicine	DHPS is an antimicrobial therapeutic target by chemical and genetic means	Burkholderia cenocepacia

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Burkholderia cenocepacia

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant apo-enzyme and enzyme in complex with inhibitor sulphadoxine or product 7,8-dihydropteroate, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 7.5 mg/ml protein in 50 mM Tris-HCl, 250 mM NaCl pH 7.5, with 0.001 ml of reservoir solution, containing 0.1 M Tris-HCl, pH 8, 10% PEG 8000, 0.3 M MgCl2, 4°C, X-ray diffraction structure determinationation and analysis at 1.95-2.35 A resolution, molecular replacement and modeling	Burkholderia cenocepacia

Crystallization (Comment)

Organism

purified recombinant apo-enzyme and enzyme in complex with inhibitor sulphadoxine or product 7,8-dihydropteroate, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 7.5 mg/ml protein in 50 mM Tris-HCl, 250 mM NaCl pH 7.5, with 0.001 ml of reservoir solution, containing 0.1 M Tris-HCl, pH 8, 10% PEG 8000, 0.3 M MgCl2, 4°C, X-ray diffraction structure determinationation and analysis at 1.95-2.35 A resolution, molecular replacement and modeling

Burkholderia cenocepacia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	recombinant enzyme, gel filtration	Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate	Burkholderia cenocepacia	-	diphosphate + 7,8-dihydropteroate	-	r

Organism

Organism	UniProt	Comment	Textmining
Burkholderia cenocepacia	A0A0H2XP43	-	-
no activity in Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography and gel filtration	Burkholderia cenocepacia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate	-	Burkholderia cenocepacia	diphosphate + 7,8-dihydropteroate	-	r
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate	-	Burkholderia cenocepacia	diphosphate + 7,8-dihydropteroate	7,8-dihydropteroate binding mode, overview	r

Subunits

Subunits	Comment	Organism
dimer	structure and sequence comparison, overview	Burkholderia cenocepacia

Synonyms

Synonyms	Comment	Organism
DHPS	-	Burkholderia cenocepacia

General Information

General Information	Comment	Organism
metabolism	the enzyme supports the biosynthesis of folate, a key metabolite required to support the synthesis of DNA, and proteins	Burkholderia cenocepacia
additional information	analysis of DHPS active site and interactions with the enzyme product 7,8-dihydropteroate, overview. DHPS shows plasticity near the substrate-binding pocket and a range of loop conformations that contribute to the architecture of the DHPS active site	Burkholderia cenocepacia