Any feedback?
Literature summary for 2.5.1.15 extracted from
- Probing the roles of non-homologous insertions in the N-terminal domain of Plasmodium falciparum hydroxymethylpterin pyrophosphokinase-dihydropteroate synthase (2009), Mol. Biochem. Parasitol., 168, 135-142.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA247-306 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are increased compared to mutant DELTA257-306 but still decreased compared to wild type, catalytic efficacy (kcat) is comparable to wild type | Plasmodium falciparum |
| DELTA257-306 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are significantly decreased compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type | Plasmodium falciparum |
| DELTA74-80 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are 2 to 3 fold higher compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type | Plasmodium falciparum |
| DELTA74-86 | no DHPS activity is detected | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
p-Aminobenzoate | mutant DELTA257-306 | Plasmodium falciparum |  |
| 0.0036 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
| 0.0039 | - |
p-Aminobenzoate | wild type | Plasmodium falciparum | |
| 0.0063 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
| 0.00708 | - |
p-Aminobenzoate | mutant DELTA74-80 | Plasmodium falciparum | |
| 0.008 | - |
p-Aminobenzoate | mutant DELTA247-306 | Plasmodium falciparum | |
| 0.0148 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum | |
| 0.0514 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Plasmodium falciparum | diphosphate + 7,8-dihydropteroate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHPS | - |
Plasmodium falciparum |
| dihydropteroate synthase | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum | |
| 0.035 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
| 0.068 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
| 0.069 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Plasmodium falciparum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.08 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
| 4.7 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum | |
| 5.86 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
| 6.23 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
