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Literature summary for 2.5.1.145 extracted from
- First ever isolation of bacterial prolipoprotein diacylglyceryl transferase in single step from Lactococcus lactis (2013), Protein Expr. Purif., 87, 120-128 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Lactococcus lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
L-1-phosphatidyl-sn-glycerol | at pH 8.0 and 37°C | Lactococcus lactis |  |
| 0.02 | - |
MKATKSAVGSTLAGCSSHHHHHH | at pH 8.0 and 37°C | Lactococcus lactis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Lactococcus lactis | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | - |
- |
- |
| Lactococcus lactis ATCC 11454 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM Sepharose column chromatography | Lactococcus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01486 | - |
unpurified enzyme, at pH 8.0 and 37°C | Lactococcus lactis |
| 0.2972 | - |
20fold purified enzyme, at pH 8.0 and 37°C | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-1-phosphatidyl-sn-glycerol + MKATKSAVGSTLAGCSSHHHHHH | - |
Lactococcus lactis | sn-glycerol 1-phosphate + MKATKSAVGSTLAG-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSHHHHHH | - |
? | |
| L-1-phosphatidyl-sn-glycerol + MKATKSAVGSTLAGCSSHHHHHH | - |
Lactococcus lactis ATCC 11454 | sn-glycerol 1-phosphate + MKATKSAVGSTLAG-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSHHHHHH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Lactococcus lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LGT | - |
Lactococcus lactis |
| phosphatidylglycerol: prolipoproteindiacylglyceryl transferase | - |
Lactococcus lactis |
| prolipoprotein diacylglyceryl transferase | - |
Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Lactococcus lactis |
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Release 2023.1 (January 2023)
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