Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli CE43 (DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with phosphatidylglycerol and palmitic acid, hanging drop vapor diffusion method, using 0.1 M Tris (pH 6.5), 0.1 M CaCl2 and 13% (w/v) PEG2000 MME | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D88N | the mutation has no effect on enzyme activity | Escherichia coli |
D97N | the mutation has no effect on enzyme activity | Escherichia coli |
E151A | inactive | Escherichia coli |
E151Q | inactive | Escherichia coli |
G138I | inactive | Escherichia coli |
G138V | inactive | Escherichia coli |
G142A | inactive | Escherichia coli |
G142I | inactive | Escherichia coli |
G142V | inactive | Escherichia coli |
G145A | inactive | Escherichia coli |
G145I | inactive | Escherichia coli |
G145V | inactive | Escherichia coli |
G27L | inactive | Escherichia coli |
G27Q | inactive | Escherichia coli |
G27W | inactive | Escherichia coli |
R143A | inactive | Escherichia coli |
Y26A | inactive | Escherichia coli |
Y26F | inactive | Escherichia coli |
Y26Q | inactive | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
n-octyl-beta-D-glucoside | - |
Escherichia coli | |
palmitic acid | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60955 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap nickel column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dipalmitoyl phosphatidic acid + MKATKLVLGAVILGSTLLAGCSSN | weak activity | Escherichia coli | ? | - |
? | |
dipalmitoyl phosphatidyl-L-serine + MKATKLVLGAVILGSTLLAGCSSN | weak activity | Escherichia coli | ? | - |
? | |
dipalmitoyl-L-1-phosphatidyl-sn-glycerol + MKATKLVLGAVILGSTLLAGCSSN | - |
Escherichia coli | dipalmitoyl-sn-glycerol 1-phosphate + MKATKLVLGAVILGSTLLAGC-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSN | - |
? | |
L-1-phosphatidyl-sn-glycerol + MKATKLVLGAVILGSTLLAGCSSN | - |
Escherichia coli | sn-glycerol 1-phosphate + MKATKLVLGAVILGSTLLAGC-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSN | - |
? | |
additional information | neutral phospholipid, dipalmitoyl phosphatidyl-ethanolamine, dipalmitoyl phosphatidyl-choline and diacylglycerol are no substrates | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGT | - |
Escherichia coli |
lipoprotein diacylglyceryl transferase | - |
Escherichia coli |
phosphatidylglycerol:prolipoproteindiacylglyceryl transferase | - |
Escherichia coli |