BRENDA - Enzyme Database
show all sequences of 2.5.1.144

Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium

Nakamura, T.; Iwahashi, H.; Eguchi, Y.; J. Bacteriol. 158, 1122-1127 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-cycloserine
18% residual activity at 5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
hydroxylamin
3% residual activity at 10 mM
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
not inhibited by L-methionine
Salmonella enterica subsp. enterica serovar Typhimurium
S-sulfocysteine
74% residual activity at 5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
Semicarbazide
40% residual activity at 1 mM
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
thiosulfate
at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
9
-
O-acetyl-L-serine
at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-acetyl-L-serine + thiosulfate
Salmonella enterica subsp. enterica serovar Typhimurium
-
S-sulfo-L-cysteine + acetate
-
-
?
O-acetyl-L-serine + thiosulfate
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
S-sulfo-L-cysteine + acetate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
P29848
-
-
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
P29848
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, DEAE-cellulose DE-32 column chromatography, DEAE-Sepharose CL-6B column chromatography, DEAE-cellulose DE-53 column chromatography, hydroxylapatite column chromatography, and Sephacryl S-200 gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.13
-
crude extract, at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
276.6
-
after 2212fold purification, at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
azaserine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
azaserine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
azaserine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
azaserine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
additional information
no activity with O-phosphoserine, O-succinylhomoserine, and L-serine
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no activity with O-phosphoserine, O-succinylhomoserine, and L-serine
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
-
O-acetyl-L-serine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
O-acetyl-L-serine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
O-acetyl-L-serine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
S-sulfo-L-cysteine + acetate
-
-
-
?
O-acetyl-L-serine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
S-sulfo-L-cysteine + acetate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Synonyms
Synonyms
Commentary
Organism
cysteine synthase B
-
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
65
77
the enzyme is inactivated by the treatment around 65 to 67.5C, and practically complete inactivation is observed at 77.5C
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-cycloserine
18% residual activity at 5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
hydroxylamin
3% residual activity at 10 mM
Salmonella enterica subsp. enterica serovar Typhimurium
additional information
not inhibited by L-methionine
Salmonella enterica subsp. enterica serovar Typhimurium
S-sulfocysteine
74% residual activity at 5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
Semicarbazide
40% residual activity at 1 mM
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
thiosulfate
at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
9
-
O-acetyl-L-serine
at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-acetyl-L-serine + thiosulfate
Salmonella enterica subsp. enterica serovar Typhimurium
-
S-sulfo-L-cysteine + acetate
-
-
?
O-acetyl-L-serine + thiosulfate
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
S-sulfo-L-cysteine + acetate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-cellulose DE-32 column chromatography, DEAE-Sepharose CL-6B column chromatography, DEAE-cellulose DE-53 column chromatography, hydroxylapatite column chromatography, and Sephacryl S-200 gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.13
-
crude extract, at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
276.6
-
after 2212fold purification, at pH 7.6, temperature not specified in the publication
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
azaserine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
azaserine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
azaserine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
azaserine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
additional information
no activity with O-phosphoserine, O-succinylhomoserine, and L-serine
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no activity with O-phosphoserine, O-succinylhomoserine, and L-serine
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
-
O-acetyl-L-serine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
O-acetyl-L-serine + Na2S
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
?
-
-
-
?
O-acetyl-L-serine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium
S-sulfo-L-cysteine + acetate
-
-
-
?
O-acetyl-L-serine + thiosulfate
-
748059
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
S-sulfo-L-cysteine + acetate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
65
77
the enzyme is inactivated by the treatment around 65 to 67.5C, and practically complete inactivation is observed at 77.5C
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749025
Gotor
S-sulfocysteine synthase func ...
Arabidopsis thaliana
Plant Signal. Behav.
8
e23313
2013
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748967
Bermudez
Photosynthetic adaptation to ...
Arabidopsis thaliana
Plant Physiol.
160
274-288
2012
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748910
Bermudez
Arabidopsis S-sulfocysteine s ...
Arabidopsis thaliana
Plant Cell
22
403-416
2010
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748059
Nakamura
Enzymatic proof for the ident ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT-2
J. Bacteriol.
158
1122-1127
1984
-
-
-
-
-
-
5
2
-
-
-
2
-
3
-
-
1
-
-
-
2
-
10
1
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
2
-
-
-
2
-
-
-
1
-
-
2
-
10
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
746931
Hensel
O-Acetylserine sulfhydrylase ...
Rhodocyclus tenuis, Rhodocyclus tenuis BN230
Arch. Microbiol.
134
227-232
1983
-
-
-
-
-
-
2
4
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
748058
Nakamura
Evidence that thiosulfate ass ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT-2
J. Bacteriol.
156
656-662
1983
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-