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Literature summary for 2.5.1.141 extracted from

  • Saiki, K.; Mogi, T.; Ogura, K.; Anraku, Y.
    In vitro heme O synthesis by the cyoE gene product from Escherichia coli (1993), J. Biol. Chem., 268, 26041-26044.
    View publication on PubMed
Search for more literature for 2.5.1.141

Activating Compound

Activating Compound Comment Organism Structure
Dithionite presence of reducing agents is essential, and dithionite is most effective among reagents tested Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protohemMe and farnesyl diphosphate to heme i n the presence of divalent cations such as Mg2+ or Ca2+ Escherichia coli
Mg2+ the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protohemMe and farnesyl diphosphate to heme i n the presence of divalent cations such as Mg2+ or Ca2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Escherichia coli
28000
-
 x * 28000, urea-SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoheme IX + (2E,6E)-farnesyl diphosphate + H2O Escherichia coli
-
 heme o + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEA5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoheme IX + (2E,6E)-farnesyl diphosphate + H2O
-
 Escherichia coli heme o + diphosphate
-
 ?
protoheme IX + (2E,6E)-farnesyl diphosphate + H2O the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protoheme and farnesyl diphosphate to heme in the presence of divalent cations such as Mg2+ or Ca2+. The CyoE protein in vitro catalyzes a direct transfer of the farnesyl moiety from a farnesyl diphosphate-Mg2+ complex to position 2 of the vinyl group at pyrrole ring D of ferrous protoheme M, possibly by a one-step reaction Escherichia coli heme o + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Escherichia coli
? x * 28000, urea-SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
cyoE
-
 Escherichia coli
heme B farnesyltransferase
-
 Escherichia coli
heme o synthase
-
 Escherichia coli
JW0418 locus name Escherichia coli