Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Humulus lupulus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type | Humulus lupulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00136 | - |
phlorisovalerophenone | truncated recombinant protein, pH 7.0, 30°C | Humulus lupulus | |
0.00339 | - |
phlorisovalerophenone | presence of prenyltransferase PT2, pH 7.0, 30°C | Humulus lupulus | |
0.0051 | - |
phlorisobutyrophenone | truncated recombinant protein, pH 7.0, 30°C | Humulus lupulus | |
0.0057 | - |
geranyl diphosphate | truncated recombinant protein, pH 7.0, 30°C | Humulus lupulus | |
0.00618 | - |
geranyl diphosphate | presence of prenyltransferase PT2, pH 7.0, 30°C | Humulus lupulus | |
0.00668 | - |
phlorisobutyrophenone | presence of prenyltransferase PT2, pH 7.0, 30°C | Humulus lupulus | |
0.0435 | - |
dimethylallyl diphosphate | truncated recombinant protein, pH 7.0, 30°C | Humulus lupulus | |
0.0436 | - |
dimethylallyl diphosphate | presence of prenyltransferase PT2, pH 7.0, 30°C | Humulus lupulus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humulus lupulus | A0A0B5A051 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + phlorisobutyrophenone | - |
Humulus lupulus | diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one | - |
? | |
dimethylallyl diphosphate + phlorisovalerophenone | - |
Humulus lupulus | diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one | - |
? | |
geranyl diphosphate + phlorisovalerophenone | - |
Humulus lupulus | diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-geranylphenyl]propan-1-one | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aromatic prenyltransferase | - |
Humulus lupulus |
PT1L | - |
Humulus lupulus |
General Information | Comment | Organism |
---|---|---|
physiological function | coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps | Humulus lupulus |