Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces hygroscopicus subsp. limoneus |
Crystallization (Comment) | Organism |
---|---|
in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7'-phosphate, and in complex with GDP and trehalose, hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 8.0 with 18-35% polyethylene glycol 3350 | Streptomyces hygroscopicus subsp. limoneus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-valienol + validamine 7-phosphate | Streptomyces hygroscopicus subsp. limoneus | - |
validoxylamine A 7'-phosphate + GDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus subsp. limoneus | Q15JG1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Superdex 200 gel filtration | Streptomyces hygroscopicus subsp. limoneus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-valienol + validamine 7-phosphate | - |
Streptomyces hygroscopicus subsp. limoneus | validoxylamine A 7'-phosphate + GDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Streptomyces hygroscopicus subsp. limoneus |
Synonyms | Comment | Organism |
---|---|---|
validamine-7-phosphate valienyltransferase | - |
Streptomyces hygroscopicus subsp. limoneus |
vldE | - |
Streptomyces hygroscopicus subsp. limoneus |