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Literature summary for 2.5.1.135 extracted from

  • Cavalier, M.C.; Yim, Y.S.; Asamizu, S.; Neau, D.; Almabruk, K.H.; Mahmud, T.; Lee, Y.H.
    Mechanistic insights into validoxylamine A 7-phosphate synthesis by VldE using the structure of the entire product complex (2012), PLoS ONE, 7, e44934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus subsp. limoneus

Crystallization (Commentary)

Crystallization (Comment) Organism
in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7'-phosphate, and in complex with GDP and trehalose, hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 8.0 with 18-35% polyethylene glycol 3350 Streptomyces hygroscopicus subsp. limoneus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-valienol + validamine 7-phosphate Streptomyces hygroscopicus subsp. limoneus
-
validoxylamine A 7'-phosphate + GDP
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus subsp. limoneus Q15JG1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Streptomyces hygroscopicus subsp. limoneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-valienol + validamine 7-phosphate
-
Streptomyces hygroscopicus subsp. limoneus validoxylamine A 7'-phosphate + GDP
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Streptomyces hygroscopicus subsp. limoneus

Synonyms

Synonyms Comment Organism
validamine-7-phosphate valienyltransferase
-
Streptomyces hygroscopicus subsp. limoneus
vldE
-
Streptomyces hygroscopicus subsp. limoneus