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Literature summary for 2.5.1.135 extracted from
- Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis (2011), J. Am. Chem. Soc., 133, 12124-12135.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces hygroscopicus subsp. limoneus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0597 | - |
GDP-valienol | at pH 7.5 and 30°C | Streptomyces hygroscopicus subsp. limoneus |  |
| 0.308 | - |
validamine 7-phosphate | at pH 7.5 and 30°C | Streptomyces hygroscopicus subsp. limoneus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-valienol + validamine 7-phosphate | Streptomyces hygroscopicus subsp. limoneus | - |
validoxylamine A 7'-phosphate + GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus subsp. limoneus | Q15JG1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Streptomyces hygroscopicus subsp. limoneus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-valienol + validamine 7-phosphate | - |
Streptomyces hygroscopicus subsp. limoneus | validoxylamine A 7'-phosphate + GDP | - |
? | |
| additional information | incubation of the enzyme with UDP- or GDP-glucose and glucose 6-phosphate does not give any product. No activity with validoxylamine A and UDP-glucose | Streptomyces hygroscopicus subsp. limoneus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pseudoglycosyltransferase | - |
Streptomyces hygroscopicus subsp. limoneus |
| vldE | - |
Streptomyces hygroscopicus subsp. limoneus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
GDP-valienol | at pH 7.5 and 30°C | Streptomyces hygroscopicus subsp. limoneus | |
| 7 | - |
validamine 7-phosphate | at pH 7.5 and 30°C | Streptomyces hygroscopicus subsp. limoneus |
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Release 2023.1 (January 2023)
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