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Literature summary for 2.5.1.122 extracted from

  • Yu, H.; Liebhold, M.; Xie, X.; Li, S.M.
    Tyrosine O-prenyltransferases TyrPT and SirD displaying similar behavior toward unnatural alkyl or benzyl diphosphate as their natural prenyl donor dimethylallyl diphosphate (2015), Appl. Microbiol. Biotechnol., 99, 7115-7124.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.13
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.17
-
dimethylallyl diphosphate pH 7.5, 37┬░C Leptosphaeria maculans
0.22
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.23
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.52
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.71
-
dimethylallyl diphosphate pH 7.5, 37┬░C Aspergillus niger
0.75
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Leptosphaeria maculans Q6Q874
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-pentenyl diphosphate + 3-fluoro-DL-tyrosine
-
Leptosphaeria maculans diphosphate + 3-fluoro-4-O-(2-pentenyl)-DL-tyrosine 20.5% conversion ?
2-pentenyl diphosphate + 3-fluoro-DL-tyrosine
-
Aspergillus niger diphosphate + 3-fluoro-4-O-(2-pentenyl)-DL-tyrosine 43.9% conversion ?
2-pentenyl diphosphate + 4-amino-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 4-(2-pentenylamino)-L-phenylalanine 42.6% conversion ?
2-pentenyl diphosphate + 4-amino-L-phenylalanine
-
Aspergillus niger diphosphate + 4-(2-pentenylamino)-L-phenylalanine 46.4% conversion ?
2-pentenyl diphosphate + alpha-methyl-L-tyrosine
-
Aspergillus niger diphosphate + alpha-methyl-4-O-(2-pentenyl)-L-tyrosine 10.3% conversion ?
2-pentenyl diphosphate + alpha-methyl-L-tyrosine
-
Leptosphaeria maculans diphosphate + alpha-methyl-4-O-(2-pentenyl)-L-tyrosine 33.5% conversion ?
2-pentenyl diphosphate + L-tyrosine
-
Leptosphaeria maculans diphosphate + 4-O-(2-pentenyl)-L-tyrosine 44.3% conversion ?
2-pentenyl diphosphate + L-tyrosine
-
Aspergillus niger diphosphate + 4-O-(2-pentenyl)-L-tyrosine 56.6% conversion ?
benzyl diphosphate + 4-amino-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 4-(benzylamino)-L-phenylalanine 54.6% conversion ?
benzyl diphosphate + 4-amino-L-phenylalanine
-
Aspergillus niger diphosphate + 4-(benzylamino)-L-phenylalanine 67.2% conversion ?
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine
-
Aspergillus niger diphosphate + 3-hydroxy-4-dimethylallyloxy-L-phenylalanine 100% conversion ?
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 3-hydroxy-4-dimethylallyloxy-L-phenylalanine 88.2% conversion ?
dimethylallyl diphosphate + 3,5-dibromo-4-hydroxy-L-phenylalanine
-
Aspergillus niger diphosphate + 3,5-dibromo-4-dimethylallyloxy-L-phenylalanine 20.6% conversion ?
dimethylallyl diphosphate + 3,5-dibromo-4-hydroxy-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 3,5-dibromo-4-dimethylallyloxy-L-phenylalanine 53.3% conversion ?
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine
-
Aspergillus niger diphosphate + 3-fluoro-4-O-dimethylallyl-DL-tyrosine 50.1% conversion ?
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine
-
Leptosphaeria maculans diphosphate + 3-fluoro-4-O-dimethylallyl-DL-tyrosine 66.5% conversion ?
dimethylallyl diphosphate + 3-iodo-L-tyrosine
-
Aspergillus niger diphosphate + 3-iodo-4-O-dimethylallyl-L-tyrosine 100% conversion ?
dimethylallyl diphosphate + 3-iodo-L-tyrosine
-
Leptosphaeria maculans diphosphate + 3-iodo-4-O-dimethylallyl-L-tyrosine 100% conversion ?
dimethylallyl diphosphate + 4-amino-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 4-(dimethylallylamino)-L-phenylalanine 66.1% conversion ?
dimethylallyl diphosphate + 4-amino-L-phenylalanine
-
Aspergillus niger diphosphate + 4-(dimethylallylamino)-L-phenylalanine 78.1% conversion ?
dimethylallyl diphosphate + alpha-methyl-L-tyrosine
-
Leptosphaeria maculans diphosphate + alpha-methyl-4-O-dimethylallyl-L-tyrosine 89.4% conversion ?
dimethylallyl diphosphate + alpha-methyl-L-tyrosine
-
Aspergillus niger diphosphate + alpha-methyl-4-O-dimethylallyl-L-tyrosine 98.3% conversion ?
dimethylallyl diphosphate + D-tyrosine
-
Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-D-tyrosine 57.6% conversion ?
dimethylallyl diphosphate + D-tyrosine
-
Aspergillus niger diphosphate + 4-O-dimethylallyl-D-tyrosine 59.5% conversion ?
dimethylallyl diphosphate + L-tyrosine
-
Aspergillus niger diphosphate + 4-O-dimethylallyl-L-tyrosine 89.1% conversion ?
dimethylallyl diphosphate + L-tyrosine
-
Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine 99.3% conversion ?
methylallyl diphosphate + 4-amino-L-phenylalanine
-
Leptosphaeria maculans diphosphate + 4-(methylallylamino)-L-phenylalanine 34.8% conversion ?
methylallyl diphosphate + 4-amino-L-phenylalanine
-
Aspergillus niger diphosphate + 4-(methylallylamino)-L-phenylalanine 57.7% conversion ?
methylallyl diphosphate + L-tyrosine
-
Leptosphaeria maculans diphosphate + 4-O-methylallyl-L-tyrosine 28% conversion ?
methylallyl diphosphate + L-tyrosine
-
Aspergillus niger diphosphate + 4-O-methylallyl-L-tyrosine 21.2% conversion ?
additional information in presence of unnatural dimethylallyl diphosphate analogs such as methylallyl, 2-pentenyl and benzyl diphosphate for alkylation, or benzylation of the indole ring enzyme catalyzes the regiospecific O- or N-alkylation or benzylation at position C4 of the aromatic ring Aspergillus niger ?
-
?
additional information in presence of unnatural dimethylallyl diphosphate analogs such as methylallyl, 2-pentenyl and benzyl diphosphate for alkylation, or benzylation of the indole ring enzyme catalyzes the regiospecific O- or N-alkylation or benzylation at position C4 of the aromatic ring Leptosphaeria maculans ?
-
?

Synonyms

Synonyms Comment Organism
SirD
-
Leptosphaeria maculans
TyrPT
-
Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.002
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.003
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.003
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.016
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.028
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.69
-
dimethylallyl diphosphate pH 7.5, 37┬░C Aspergillus niger
1
-
dimethylallyl diphosphate pH 7.5, 37┬░C Leptosphaeria maculans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.021
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.031
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.037
-
benzyl diphosphate cosubstrate 4-aminophenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
0.069
-
methylallyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Aspergillus niger
0.098
-
2-pentenyl diphosphate cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37┬░C Leptosphaeria maculans
5.88
-
dimethylallyl diphosphate pH 7.5, 37┬░C Leptosphaeria maculans