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Literature summary for 2.5.1.122 extracted from

  • Kremer, A.; Li, S.M.
    A tyrosine O-prenyltransferase catalyses the first pathway-specific step in the biosynthesis of sirodesmin PL (2010), Microbiology, 156, 278-286.
    View publication on PubMed
Search for more literature for 2.5.1.122

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Leptosphaeria maculans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 12% inhibition Leptosphaeria maculans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 L-tyrosine pH 7.5, 37°C Leptosphaeria maculans
0.17
-
 dimethylallyl diphosphate pH 7.5, 37°C Leptosphaeria maculans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 1.4fold activation compared to activity without additives Leptosphaeria maculans
additional information no requirement for divalent metal ion Leptosphaeria maculans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Leptosphaeria maculans
100000
-
 His6-tagged enzyme, gel filtration Leptosphaeria maculans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tyrosine Leptosphaeria maculans the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?

Organism

Organism UniProt Comment Textmining
Leptosphaeria maculans Q6Q874
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leptosphaeria maculans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-3,4-dihydroxyphenylalanine 16.6% of the activity, compared to L-tyrosine. It is expected that the prenylation takes place at the 4-hydroxyl group. The structure of the product is not determined Leptosphaeria maculans ?
-
 ?
dimethylallyl diphosphate + L-tyrosine the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
dimethylallyl diphosphate + L-tyrosine no activity with cyclo-L-Tyr-L-Ser, L-Phe, 4-hydroxybenzoate or 4-coumarate Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
additional information SirD also prenylates L-Trp, resulting in the formation of 7-dimethylallyltryptophan. SirD catalyses the C-prenylation of L-Trp, in addition to the O-prenylation of L-Tyr, cf. EC 2.5.1.34 Leptosphaeria maculans ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Leptosphaeria maculans

Synonyms

Synonyms Comment Organism
SirD
-
 Leptosphaeria maculans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Leptosphaeria maculans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 L-tyrosine pH 7.5, 37°C Leptosphaeria maculans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Leptosphaeria maculans

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of the epidithiopiperazinedione toxin sirodesmin PL by the phytopathogenic ascomycete Leptosphaeria maculans Leptosphaeria maculans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.7
-
 L-tyrosine pH 7.5, 37°C Leptosphaeria maculans