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Literature summary for 2.5.1.121 extracted from

  • Saleh, O.; Gust, B.; Boll, B.; Fiedler, H.P.; Heide, L.
    Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-carboxylate dimethylallyltransferase from Streptomyces anulatus (2009), J. Biol. Chem., 284, 14439-14447.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.121

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, increases reaction velocity 1.5fold Streptomyces anulatus

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Streptomyces coelicolor M512 Streptomyces anulatus

Inhibitors

Inhibitors Comment Organism Structure
FeSO4 10 mM, completely abolishes the formation of endophenazine A Streptomyces anulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus
0.116
-
 dimethylallyl diphosphate pH 7.5, 30°C Streptomyces anulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 100 mM MgCl2 increases product formation 1.5fold Streptomyces anulatus
Mg2+ the enzyme is independent of the presence of magnesium or other divalent cations. 50 mM MgCl2 increases product formation 1.5fold Streptomyces anulatus
Na+ 500 mM, NaCl increases product formation 3fold Streptomyces anulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Streptomyces anulatus
37138
-
 x * 37138, calculated from sequence Streptomyces anulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus the enzyme is involved in the biosynthesis of prenylated phenazines diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus 9663 the enzyme is involved in the biosynthesis of prenylated phenazines diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces anulatus C4PWA1
-
-
Streptomyces anulatus 9663 C4PWA1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces anulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus 9663 diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus 9663 diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Streptomyces anulatus
? x * 37138, calculated from sequence Streptomyces anulatus

Synonyms

Synonyms Comment Organism
5,10-dihydrophenazine 1-carboxylate dimethylallyltransferase
-
 Streptomyces anulatus
dihydrophenazine-1-carboxylate dimethylallyltransferase
-
 Streptomyces anulatus
Ppzp
-
 Streptomyces anulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.435
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces anulatus

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 pH 5.0: about 50% of maximal activity, ph 9.5: about 50% of maximal activity Streptomyces anulatus

General Information

General Information Comment Organism
malfunction after inactivation of ppzP, only nonprenylated phenazine 1-carboxylic acid is formed Streptomyces anulatus
physiological function the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.4
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus