Literature summary for 2.5.1.114 extracted from

Umitsu, M.; Nishimasu, H.; Noma, A.; Suzuki, T.; Ishitani, R.; Nureki, O.
Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2009), Proc. Natl. Acad. Sci. USA, 106, 15616-15621.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Pyrococcus horikoshii
-	Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the enzyme-S-adenosyl-L-methionine complex are determined at 2.0 A resolution	Methanocaldococcus jannaschii
crystals of the enzyme-S-adenosyl-L-methionine complex are grown from the reservoir solution containing 0.1 M Mes-NaOH, pH 6.0, and 14% PEG 10000. The crystals contain one PhTYW2 monomer with one AdoMet or MeSAdo molecule per asymmetric unit, and belong to the space group R3	Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	Pyrococcus horikoshii	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	Methanocaldococcus jannaschii	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	Q58952	-	-
Pyrococcus horikoshii	O58523	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii
-	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	Pyrococcus horikoshii	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	Methanocaldococcus jannaschii	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	analysis of products by mass spectrometric analysis	Pyrococcus horikoshii	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe	analysis of products by mass spectrometric analysis	Methanocaldococcus jannaschii	S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe	-	?

Synonyms

Synonyms	Comment	Organism
MJ1557	-	Methanocaldococcus jannaschii
PH0793	-	Pyrococcus horikoshii
TYW2	-	Pyrococcus horikoshii
TYW2	-	Methanocaldococcus jannaschii

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	Pyrococcus horikoshii
physiological function	the enzyme is involved in the biosynthesis of the tricyclic base wybutosine	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)