Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
- |
Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
crystals of the enzyme-S-adenosyl-L-methionine complex are determined at 2.0 A resolution | Methanocaldococcus jannaschii |
crystals of the enzyme-S-adenosyl-L-methionine complex are grown from the reservoir solution containing 0.1 M Mes-NaOH, pH 6.0, and 14% PEG 10000. The crystals contain one PhTYW2 monomer with one AdoMet or MeSAdo molecule per asymmetric unit, and belong to the space group R3 | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | Pyrococcus horikoshii | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | Methanocaldococcus jannaschii | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58952 | - |
- |
Pyrococcus horikoshii | O58523 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Pyrococcus horikoshii | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Methanocaldococcus jannaschii | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | analysis of products by mass spectrometric analysis | Pyrococcus horikoshii | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe | analysis of products by mass spectrometric analysis | Methanocaldococcus jannaschii | S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MJ1557 | - |
Methanocaldococcus jannaschii |
PH0793 | - |
Pyrococcus horikoshii |
TYW2 | - |
Pyrococcus horikoshii |
TYW2 | - |
Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Pyrococcus horikoshii |
physiological function | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Methanocaldococcus jannaschii |