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Literature summary for 2.5.1.113 extracted from

  • Jurgenson, C.T.; Burns, K.E.; Begley, T.P.; Ealick, S.E.
    Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis (2008), Biochemistry, 47, 10354-10364.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) and BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis H37Rv
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis H37Rv [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CysM
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis