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Literature summary for 2.5.1.113 extracted from

  • OLeary, S.E.; Jurgenson, C.T.; Ealick, S.E.; Begley, T.P.
    O-Phospho-l-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis (2008), Biochemistry, 47, 11606-11615.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
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-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH the specificity of the enzyme for its amino acid substrate is more than 500fold greater for O-phospho-L-serine than for O-acetyl-L-serine Mycobacterium tuberculosis ?
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 ?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide Mycobacterium tuberculosis [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
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 ?

Synonyms

Synonyms Comment Organism
CysM
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 Mycobacterium tuberculosis
Cysteine synthase
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 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis