Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Aspergillus sp. MF297-2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + brevianamide F | Aspergillus sp. MF297-2 | - |
diphosphate + deoxybrevianamide E | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. MF297-2 | E0Y3X1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Aspergillus sp. MF297-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + brevianamide F | - |
Aspergillus sp. MF297-2 | diphosphate + deoxybrevianamide E | - |
? | |
dimethylallyl diphosphate + brevianamide F | i.e. cyclo-L-Trp-L-Pro, conversion yield of 66% | Aspergillus sp. MF297-2 | diphosphate + deoxybrevianamide E | - |
? | |
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala | conversion yield of 1% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala | conversion yield of less than 3% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro | conversion yield of 6% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala | conversion yield of 17% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro | conversion yield of 18% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala | conversion yield of 5% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro | conversion yield of 61% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-Gly | conversion yield of 6% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala | conversion yield of 11% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-L-His | conversion yield of less than 3% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-L-Phe | conversion yield of less than 3% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | conversion yield of less than 3% | Aspergillus sp. MF297-2 | ? | - |
? | |
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr | conversion yield of less than 3% | Aspergillus sp. MF297-2 | ? | - |
? | |
additional information | the enzyme shows no activity with L-Trp, cyclo-L-Trp-L-Trp, cyclo-L-Phe-L-Pro and cyclo-L-Trp-L-Tyr | Aspergillus sp. MF297-2 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Aspergillus sp. MF297-2 |
Synonyms | Comment | Organism |
---|---|---|
NotF | - |
Aspergillus sp. MF297-2 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of notoamides | Aspergillus sp. MF297-2 |