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Literature summary for 2.5.1.106 extracted from
- Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Aspergillus fumigatus (2005), Microbiology, 151, 2199-2207.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | at 2-5 mM, slight activation. Inhibitory above 10 mM | Aspergillus fumigatus |  |
| Mn2+ | inhibitory above 5 mM | Aspergillus fumigatus | |
| additional information | not inhibitory: EDTA up to 5 mM | Aspergillus fumigatus | |
| Zn2+ | inhibitory above 5 mM | Aspergillus fumigatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
brevianamide F | pH 7.5, 30°C | Aspergillus fumigatus | |
| 0.074 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 2-5 mM, slight activation | Aspergillus fumigatus | |
| Mg2+ | at 2-5 mM, slight activation. Inhibitory above 10 mM | Aspergillus fumigatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
| 53400 | - |
1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
| 66000 | - |
gel filtration | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q4WAW7 | - |
- |
| Aspergillus fumigatus Af293 | Q4WAW7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + brevianamide F | catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus | Aspergillus fumigatus | diphosphate + tryprostatin B | - |
? | |
| dimethylallyl diphosphate + brevianamide F | catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus | Aspergillus fumigatus Af293 | diphosphate + tryprostatin B | - |
? | |
| dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) | 32.6% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro) | - |
? | |
| dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) | 32.6% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-Gly) | 4.7% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-Gly) | 4.7% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) | 21.9% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) | 21.9% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-L-Phe) | 3.4% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Phe) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-L-Trp) | 6.4% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Trp) | - |
? | |
| dimethylallyl diphosphate + cyclo-(L-Trp-L-Tyr) | 5.0% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Tyr) | - |
? | |
| additional information | enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate | Aspergillus fumigatus | ? | - |
? | |
| additional information | enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate | Aspergillus fumigatus Af293 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FtmPT1 | - |
Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.57 | - |
brevianamide F | pH 7.5, 30°C | Aspergillus fumigatus | |
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