Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, 1.8 A resolution | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
agmatine | pH 7.4, 70°C | Pyrococcus furiosus | |
1.88 | - |
propane-1,3-diamine | pH 7.4, 70°C | Pyrococcus furiosus | |
3.77 | - |
cadaverine | pH 7.4, 70°C | Pyrococcus furiosus | |
7.7 | - |
putrescine | pH 7.4, 70°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32334 | - |
2 * 32334, calculated from sequence | Pyrococcus furiosus |
32600 | - |
2 * 32600, SDS-PAGE | Pyrococcus furiosus |
65000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + agmatine | Pyrococcus furiosus | agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required | 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + cadaverine | Pyrococcus furiosus | agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required | 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U4G1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + agmatine | agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + agmatine | 4.9% of the activity compared to cadaverine | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + cadaverine | - |
Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + cadaverine | agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | 2.9% of the activity compared to cadaverine | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + putrescine | 8.7% of the activity compared to cadaverine | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
S-adenosyl 3-(methylthio)propylamine + spermidine | 0.8% of the activity compared to cadaverine | Pyrococcus furiosus | 5'-S-methyl-5'-thioadenosine + spermine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32600, SDS-PAGE | Pyrococcus furiosus |
homodimer | 2 * 32334, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
ACAPT | - |
Pyrococcus furiosus |
agmatine/cadaverine aminopropyl transferase | - |
Pyrococcus furiosus |
PF0127 | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
90 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 103 | 70°C: about 50% of maximal activity, 103°C: about 50% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
108 | - |
transition temperature | Pyrococcus furiosus |
112 | - |
transition temperature in presence of 5 mM cadaverine | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
propane-1,3-diamine | pH 7.4, 70°C | Pyrococcus furiosus | |
0.1 | - |
agmatine | pH 7.4, 70°C | Pyrococcus furiosus | |
0.18 | - |
putrescine | pH 7.4, 70°C | Pyrococcus furiosus | |
2.09 | - |
cadaverine | pH 7.4, 70°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
putrescine | pH 7.4, 70°C | Pyrococcus furiosus | |
0.032 | - |
propane-1,3-diamine | pH 7.4, 70°C | Pyrococcus furiosus | |
0.55 | - |
cadaverine | pH 7.4, 70°C | Pyrococcus furiosus | |
13.4 | - |
agmatine | pH 7.4, 70°C | Pyrococcus furiosus |