Cloned (Comment) | Organism |
---|---|
gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 90% inhibition at 5 mM | Aspergillus fumigatus | |
Zn2+ | 90% inhibition at 5 mM | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
fumigaclavine A | pH 7.5, 30°C | Aspergillus fumigatus | |
0.013 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Aspergillus fumigatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant effects by Mg2+, Ca2+, and EDTA at 5 mM | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
recombinant His6-tagged FgaPT1, gel filtration | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumigaclavine A + dimethylallyl diphosphate | Aspergillus fumigatus | - |
fumigaclavine C + diphosphate | - |
? | |
fumigaclavine A + dimethylallyl diphosphate | Aspergillus fumigatus Af293 | - |
fumigaclavine C + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
gene fgaPT1 | - |
Aspergillus fumigatus Af293 | - |
gene fgaPT1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumigaclavine A + dimethylallyl diphosphate | - |
Aspergillus fumigatus | fumigaclavine C + diphosphate | - |
? | |
fumigaclavine A + dimethylallyl diphosphate | the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus | Aspergillus fumigatus | fumigaclavine C + diphosphate | - |
? | |
fumigaclavine A + dimethylallyl diphosphate | - |
Aspergillus fumigatus Af293 | fumigaclavine C + diphosphate | - |
? | |
fumigaclavine A + dimethylallyl diphosphate | the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus | Aspergillus fumigatus Af293 | fumigaclavine C + diphosphate | - |
? | |
additional information | FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A | Aspergillus fumigatus | ? | - |
? | |
additional information | FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A | Aspergillus fumigatus Af293 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
FgaPT1 | - |
Aspergillus fumigatus |
fumigaclavine C synthase | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus | |
0.8 | - |
fumigaclavine A | pH 7.5, 30°C | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
evolution | FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus | Aspergillus fumigatus |