Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.10 extracted from

  • Sun, X.F.; Li, Z.X.
    In silico and in vitro analyses identified three amino acid residues critical to the catalysis of two aphid farnesyl diphosphate synthase (2012), Protein J., 31, 417-424.
    View publication on PubMed
Search for more literature for 2.5.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhopalosiphum padi

Protein Variants

Protein Variants Comment Organism
K266I the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
K266I the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi
R120G the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
R120G the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi
R121G the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
R121G the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 2 * 45500, isoform FPPS1, calculated from amino acid sequence Rhopalosiphum padi
45900
-
 2 * 45900, isoform FPPS2, calculated from amino acid sequence Rhopalosiphum padi

Organism

Organism UniProt Comment Textmining
Rhopalosiphum padi G0YZR3 isoform FPPS1; bird cherry-oat aphid
-
Rhopalosiphum padi G0YZR4 isoform FPPS2; bird cherry-oat aphid
-

Purification (Commentary)

Purification (Comment) Organism
TALON resin Co2+ metal affinity column chromatography Rhopalosiphum padi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Rhopalosiphum padi 2 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45500, isoform FPPS1, calculated from amino acid sequence Rhopalosiphum padi
homodimer 2 * 45900, isoform FPPS2, calculated from amino acid sequence Rhopalosiphum padi

Synonyms

Synonyms Comment Organism
farnesyl diphosphate synthase 1
-
 Rhopalosiphum padi
farnesyl diphosphate synthase 2
-
 Rhopalosiphum padi
FPPS1 isoform Rhopalosiphum padi
FPPS2 isoform Rhopalosiphum padi

pI Value

Organism Comment pI Value Maximum pI Value
Rhopalosiphum padi isoform FPPS2, calculated from amino acid sequence
-
 6.6
Rhopalosiphum padi isoform FPPS1, calculated from amino acid sequence
-
 7.5