Literature summary for 2.5.1.1 extracted from

Yang, L.; Jiang, L.; Li, W.; Yang, Y.; Zhang, G.; Luo, Y.
A homomeric geranyl diphosphate synthase-encoding gene from Camptotheca acuminata and its combinatorial optimization for production of geraniol in Escherichia coli (2017), J. Ind. Microbiol. Biotechnol., 44, 1431-1441 .

Cloned(Commentary)

Cloned (Comment)	Organism
a homomeric geranyl-diphosphate synthase from Camptotheca acuminata, a camptothecin-producing plant, is obtained from 5'- and 3'-rapid amplification of cDNA ends and subsequent overlap extension and convenient PCR amplifications. The truncate enzyme (tCaGPPS) is introduced to replace ispA of pBbA5c-MevT(CO)-MBIS(CO, ispA), a de novo biosynthetic construct for farnesyl diphosphate generation, and overexpressed in Escherichia coli together with the truncated geraniol synthase-encoding gene (tCaGES) from Camptotheca acuminata, to confirm geranyl-diphosphate synthase-catalyzed reaction in vivo. The tCaGPPS and tCaGES genes with different copy numbers are introduced into Escherichia coli to balance their catalytic potential for high-yield geraniol production. A 1.6fold increase of geraniol production is obtained when four copies of tCaGPPS and one copy of tCaGES are introduced into Escherichia coli. The following fermentation conditions optimization, including removal of organic layers and addition of new n-decane, leads to a 74.6 mg/l of geraniol production	Camptotheca acuminata

Cloned (Comment)

Organism

a homomeric geranyl-diphosphate synthase from Camptotheca acuminata, a camptothecin-producing plant, is obtained from 5'- and 3'-rapid amplification of cDNA ends and subsequent overlap extension and convenient PCR amplifications. The truncate enzyme (tCaGPPS) is introduced to replace ispA of pBbA5c-MevT(CO)-MBIS(CO, ispA), a de novo biosynthetic construct for farnesyl diphosphate generation, and overexpressed in Escherichia coli together with the truncated geraniol synthase-encoding gene (tCaGES) from Camptotheca acuminata, to confirm geranyl-diphosphate synthase-catalyzed reaction in vivo. The tCaGPPS and tCaGES genes with different copy numbers are introduced into Escherichia coli to balance their catalytic potential for high-yield geraniol production. A 1.6fold increase of geraniol production is obtained when four copies of tCaGPPS and one copy of tCaGES are introduced into Escherichia coli. The following fermentation conditions optimization, including removal of organic layers and addition of new n-decane, leads to a 74.6 mg/l of geraniol production

Camptotheca acuminata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dimethylallyl diphosphate + isopentenyl diphosphate	Camptotheca acuminata	-	diphosphate + geranyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Camptotheca acuminata	A0A2H4ULH9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dimethylallyl diphosphate + isopentenyl diphosphate	-	Camptotheca acuminata	diphosphate + geranyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
GPP synthase	-	Camptotheca acuminata
GPPS	-	Camptotheca acuminata