Literature summary for 2.5.1.1 extracted from

Heider, S.A.; Peters-Wendisch, P.; Beekwilder, J.; Wendisch, V.F.
IdsA is the major geranylgeranyl pyrophosphate synthase involved in carotenogenesis in Corynebacterium glutamicum (2014), FEBS J., 281, 4906-4920.

Search for more literature for 2.5.1.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	isopentenyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
0.008	-	dimethylallyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
0.008	-	geranyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
0.02	-	farnesyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39600	-	-	Corynebacterium glutamicum
158000	-	gel filtration	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dimethylallyl diphosphate + isopentenyl diphosphate	Corynebacterium glutamicum	highest catalytic efficiency	diphosphate + geranyl diphosphate	-	r
dimethylallyl diphosphate + isopentenyl diphosphate	Corynebacterium glutamicum ATCC 13032	highest catalytic efficiency	diphosphate + geranyl diphosphate	-	r
diphosphate + geranyl diphosphate	Corynebacterium glutamicum	-	dimethylallyl diphosphate + isopentenyl diphosphate	-	r
diphosphate + geranyl diphosphate	Corynebacterium glutamicum ATCC 13032	-	dimethylallyl diphosphate + isopentenyl diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	-	-
Corynebacterium glutamicum ATCC 13032	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dimethylallyl diphosphate + isopentenyl diphosphate	highest catalytic efficiency	Corynebacterium glutamicum	diphosphate + geranyl diphosphate	-	r
dimethylallyl diphosphate + isopentenyl diphosphate	highest catalytic efficiency	Corynebacterium glutamicum ATCC 13032	diphosphate + geranyl diphosphate	-	r
diphosphate + farnesyl diphosphate	-	Corynebacterium glutamicum	?	-	r
diphosphate + farnesyl diphosphate	-	Corynebacterium glutamicum ATCC 13032	?	-	r
diphosphate + geranyl diphosphate	-	Corynebacterium glutamicum	dimethylallyl diphosphate + isopentenyl diphosphate	-	r
diphosphate + geranyl diphosphate	-	Corynebacterium glutamicum ATCC 13032	dimethylallyl diphosphate + isopentenyl diphosphate	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 39600, SDS-PAGE	Corynebacterium glutamicum

Synonyms

Synonyms	Comment	Organism
geranylgeranyl pyrophosphate synthase	-	Corynebacterium glutamicum
GPP (C5) synthase	-	Corynebacterium glutamicum
IdsA	-	Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	35	-	Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	the enzyme is stable at temperature below 50°C, while after 1 h at 40°C, the enzyme activity is reduced by about 80%	Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.1	-	geranyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
0.4	-	farnesyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
1.1	-	isopentenyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
1.1	-	dimethylallyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9	-	geranyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
34	-	farnesyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
140	-	isopentenyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum
140	-	dimethylallyl diphosphate	at pH 7.5 and 30°C	Corynebacterium glutamicum

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Release 2023.1 (January 2023)