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Literature summary for 2.4.99.B5 extracted from

  • Hemker, M.; Stratmann, A.; Goeke, K.; Schröder, W.; Lenz, J.; Piepersberg, W.; Pape, H.
    Identification, cloning, expression, and characterization of the extracellular acarbose-modifying glycosyltransferase, AcbD, from Actinoplanes sp. strain SE50 (2001), J. Bacteriol., 183, 4484-4492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.99.B5

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Streptomyces lividans TK23 Actinoplanes sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Actinoplanes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
 acarbose pH 6.3, 30°C Actinoplanes sp.
0.96
-
 maltose pH 6.3, 30°C Actinoplanes sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Actinoplanes sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Actinoplanes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, SDS-PAGE Actinoplanes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acarbose + maltotriose Actinoplanes sp. the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds acarviosyl-alpha-(1-4)-maltotriose + maltose
-
 ?
acarbose + maltotriose Actinoplanes sp. 50/110 the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds acarviosyl-alpha-(1-4)-maltotriose + maltose
-
 ?

Organism

Organism UniProt Comment Textmining
Actinoplanes sp. Q27GR5
-
-
Actinoplanes sp. 50/110 Q27GR5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinoplanes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + cellobiose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-cellobiose + maltose
-
 ?
acarbose + cellobiose
-
 Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-cellobiose + maltose
-
 ?
acarbose + laminaribiose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
 ?
acarbose + laminaribiose
-
 Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
 ?
acarbose + maltoheptaose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
 ?
acarbose + maltoheptaose
-
 Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
 ?
acarbose + maltohexaose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-maltohexaose + maltose
-
 ?
acarbose + maltopentaose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-maltopentaose + maltose
-
 ?
acarbose + maltose
-
 Actinoplanes sp. acarviosyl-alpha-(1->4)-maltose + maltose
-
 ?
acarbose + maltotetraose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-maltotetraose + maltose
-
 ?
acarbose + maltotriose the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds Actinoplanes sp. acarviosyl-alpha-(1-4)-maltotriose + maltose
-
 ?
acarbose + maltotriose the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds Actinoplanes sp. 50/110 acarviosyl-alpha-(1-4)-maltotriose + maltose
-
 ?
acarbose + maltotriose
-
 Actinoplanes sp. acarviosyl-alpha-(1->4)-maltotriose + maltose
-
 ?
acarbose + nonyl alpha-D-glucopyranoside
-
 Actinoplanes sp. nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose
-
 ?
acarbose + xylobiose
-
 Actinoplanes sp. acarviosyl-(1->4)-alpha-xylobiose + maltose
-
 ?
acarviosyl glucose + maltose
-
 Actinoplanes sp. acarviosyl-alpha-(1->4)-maltose + D-glucose
-
 ?
acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose
-
 Actinoplanes sp. acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc
-
 ?
additional information a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity Actinoplanes sp. ?
-
 ?
additional information a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity Actinoplanes sp. 50/110 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Actinoplanes sp.

Synonyms

Synonyms Comment Organism
AcbD
-
 Actinoplanes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Actinoplanes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 thermostable up to 40°C Actinoplanes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.9
-
 Actinoplanes sp.

pI Value

Organism Comment pI Value Maximum pI Value
Actinoplanes sp. isoelectric focusing, pH gradient from 3 to 10
-
 5