BRENDA - Enzyme Database show
show all sequences of 2.4.99.4

Reconstitution of a porcine submaxillary gland beta-D-galactoside alpha 2-3 sialyltransferase into liposomes

Westcott, K.R.; Hill, R.L.; J. Biol. Chem. 260, 13116-13121 (1985)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
lysophosphatidylcholine
C16-18 acyl derivatives of lysophosphatidylcholine stimulate the activity of enzyme form A more extensively than the C14 acyl derivative, and the C12 acyl derivative is without effect on enzyme form A
Sus scrofa
phospholipid/octylglucoside liposomes
incorporation into phospholipid/octylglucoside liposomes activates enzyme form A
Sus scrofa
Triton X-100
stimulates activity of enzyme form A. Enzyme form B is fully active in absence of detergents. Detergents stimulate the activity of enzyme form A by lowering the KD and KM of CMP-N-acetylneuraminate and increasing the Vmax of the reaction
Sus scrofa
Inhibitors
Inhibitors
Commentary
Organism
Structure
lysophosphatidylglycerol
inhibition of enzyme form A and B
Sus scrofa
lysophosphatidylserine
inhibition of enzyme form A
Sus scrofa
octyl-D-glucoside
inhibition of enzyme form A and B
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
-
Sus scrofa
5794
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
enzyme form B
Sus scrofa
49000
-
enzyme form A
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
Q02745
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
submandibular gland
-
Sus scrofa
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
lysophosphatidylcholine
C16-18 acyl derivatives of lysophosphatidylcholine stimulate the activity of enzyme form A more extensively than the C14 acyl derivative, and the C12 acyl derivative is without effect on enzyme form A
Sus scrofa
phospholipid/octylglucoside liposomes
incorporation into phospholipid/octylglucoside liposomes activates enzyme form A
Sus scrofa
Triton X-100
stimulates activity of enzyme form A. Enzyme form B is fully active in absence of detergents. Detergents stimulate the activity of enzyme form A by lowering the KD and KM of CMP-N-acetylneuraminate and increasing the Vmax of the reaction
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
lysophosphatidylglycerol
inhibition of enzyme form A and B
Sus scrofa
lysophosphatidylserine
inhibition of enzyme form A
Sus scrofa
octyl-D-glucoside
inhibition of enzyme form A and B
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
-
Sus scrofa
5794
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
enzyme form B
Sus scrofa
49000
-
enzyme form A
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
submandibular gland
-
Sus scrofa
-
Other publictions for EC 2.4.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736507
Lu
Expression of N-acetylglucosam ...
Homo sapiens
J. Biol. Chem.
291
5708-5720
2016
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723156
Sugiarto
Decreasing the sialidase activ ...
Pasteurella multocida
Mol. Biosyst.
7
3021-3027
2011
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723519
Perez-Garay
alpha2,3-Sialyltransferase ST3 ...
Homo sapiens
PLoS ONE
5
e12524
2010
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1
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705677
Huang
Human beta-galactoside alpha-2 ...
Homo sapiens
Mol. Cell. Biochem.
331
81-88
2009
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1
1
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1
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744308
Chandrasekaran
Reversible sialylation synthe ...
Rattus norvegicus
Biochemistry
47
320-330
2008
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1
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746526
Sengupta
-
Chemoenzymatic synthesis of s ...
Rattus norvegicus
Tetrahedron Lett.
44
6037-6042
2003
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1
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744624
Shigeta
alpha-(2->3)- and alpha-(2->6 ...
Mus musculus
Carbohydr. Res.
264
111-121
1994
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745255
Higgins
Aberrant O-linked oligosaccha ...
Homo sapiens
J. Biol. Chem.
266
6280-6290
1991
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745253
Westcott
Reconstitution of a porcine s ...
Sus scrofa
J. Biol. Chem.
260
13116-13121
1985
3
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3
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1
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745248
Sadler
Purification to homogeneity o ...
Sus scrofa
J. Biol. Chem.
254
4434-4443
1979
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3
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745249
Rearick
Enzymatic characterization of ...
Sus scrofa
J. Biol. Chem.
254
4444-4451
1979
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