BRENDA - Enzyme Database show
show all sequences of 2.4.99.4

Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland

Rearick, J.I.; Sadler, J.E.; Paulson, J.C.; Hill, R.L.; J. Biol. Chem. 254, 4444-4451 (1979)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
beta-D-Gal-(1->3)-beta-D-GalNAc
pH 6.5, 37°C, enzyme form A
Sus scrofa
0.21
-
beta-D-Gal-(1->3)-beta-D-GalNAc
pH 6.5, 37°C, enzyme form B
Sus scrofa
0.35
-
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form A
Sus scrofa
0.39
-
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form B
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtrazion, enzyme form A and B
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Sus scrofa
-
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
Q02745
-
-
Purification (Commentary)
Commentary
Organism
purification of enzyme form A and B
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
submandibular gland
-
Sus scrofa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
745249
Sus scrofa
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetylneuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc
best acceptor substrate
745249
Sus scrofa
CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
-
-
?
additional information
with glycoprotein and ganglioside acceptors the substrate specificity is strict, with the sequence beta-D-Gal-(1->3)-alpha-D-GalNAc serving as the exclusive acceptor. The enzyme has an equilibrium random order mechanism
745249
Sus scrofa
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
beta-D-Gal-(1->3)-beta-D-GalNAc
pH 6.5, 37°C, enzyme form A
Sus scrofa
0.21
-
beta-D-Gal-(1->3)-beta-D-GalNAc
pH 6.5, 37°C, enzyme form B
Sus scrofa
0.35
-
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form A
Sus scrofa
0.39
-
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form B
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtrazion, enzyme form A and B
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Sus scrofa
-
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Sus scrofa
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
purification of enzyme form A and B
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
submandibular gland
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
745249
Sus scrofa
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
745249
Sus scrofa
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
-
-
?
CMP-N-acetylneuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc
best acceptor substrate
745249
Sus scrofa
CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
-
-
?
additional information
with glycoprotein and ganglioside acceptors the substrate specificity is strict, with the sequence beta-D-Gal-(1->3)-alpha-D-GalNAc serving as the exclusive acceptor. The enzyme has an equilibrium random order mechanism
745249
Sus scrofa
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Sus scrofa
General Information
General Information
Commentary
Organism
metabolism
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Sus scrofa
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Sus scrofa
Other publictions for EC 2.4.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736507
Lu
Expression of N-acetylglucosam ...
Homo sapiens
J. Biol. Chem.
291
5708-5720
2016
-
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1
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1
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1
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723156
Sugiarto
Decreasing the sialidase activ ...
Pasteurella multocida
Mol. Biosyst.
7
3021-3027
2011
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1
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3
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8
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1
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1
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2
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8
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1
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1
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3
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8
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1
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2
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8
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1
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8
8
723519
Perez-Garay
alpha2,3-Sialyltransferase ST3 ...
Homo sapiens
PLoS ONE
5
e12524
2010
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1
1
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705677
Huang
Human beta-galactoside alpha-2 ...
Homo sapiens
Mol. Cell. Biochem.
331
81-88
2009
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1
1
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2
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2
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1
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2
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1
1
1
1
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-
744308
Chandrasekaran
Reversible sialylation synthe ...
Rattus norvegicus
Biochemistry
47
320-330
2008
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1
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1
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746526
Sengupta
-
Chemoenzymatic synthesis of s ...
Rattus norvegicus
Tetrahedron Lett.
44
6037-6042
2003
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1
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1
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1
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744624
Shigeta
alpha-(2->3)- and alpha-(2->6 ...
Mus musculus
Carbohydr. Res.
264
111-121
1994
-
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1
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1
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1
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1
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1
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-
745255
Higgins
Aberrant O-linked oligosaccha ...
Homo sapiens
J. Biol. Chem.
266
6280-6290
1991
-
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-
-
-
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1
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1
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1
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2
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1
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1
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2
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1
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-
745253
Westcott
Reconstitution of a porcine s ...
Sus scrofa
J. Biol. Chem.
260
13116-13121
1985
3
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3
-
1
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2
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1
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1
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1
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3
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3
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1
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2
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1
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1
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-
-
745248
Sadler
Purification to homogeneity o ...
Sus scrofa
J. Biol. Chem.
254
4434-4443
1979
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3
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1
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2
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1
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745249
Rearick
Enzymatic characterization of ...
Sus scrofa
J. Biol. Chem.
254
4444-4451
1979
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4
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1
4
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1
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1
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7
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1
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1
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4
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1
4
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1
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1
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7
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1
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1
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