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Literature summary for 2.4.99.28 extracted from
- Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase (1984), J. Biol. Chem., 259, 13937-13946.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| benzylpenicillin | stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate | Escherichia coli |  |
| Dimethylsulfoxide | activation, in the absence of methanol, inhibits in the presence of 0.05% sarkosyl | Escherichia coli | |
| EDTA | stimulation in the presence of high concentrations of methanol and detergents | Escherichia coli | |
| Imipenem | stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate | Escherichia coli | |
| additional information | no activation by cephalexin, nocardicin A or mecillinam | Escherichia coli | |
| Sarkosyl | activation | Escherichia coli | |
| Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid | stimulation in the presence of high concentrations of methanol and detergents | Escherichia coli | |
| sodium deoxycholate | 0.05-0.1%, activation | Escherichia coli | |
| Triton X-100 | activation, 0.05% | Escherichia coli | |
| Triton X-100 | inhibits at 0.1% | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli structural gene mrcB, recloned from plasmid pLC19-19 to high copy number plasmid pBr322, yielding plasmid pTM13 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli | |
| Co2+ | - |
Escherichia coli | |
| Dimethylsulfoxide | in the presence of 0.05% N-lauroylsarcosine | Escherichia coli | |
| EDTA | in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents | Escherichia coli | |
| enramycin | - |
Escherichia coli | |
| Fe2+ | - |
Escherichia coli | |
| macarbomycin | - |
Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| Moenomycin | - |
Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid | in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents | Escherichia coli | |
| sodium deoxycholate | in the presence of methanol, inhibits at 0.5% | Escherichia coli | |
| Triton X-100 | inhibits at 0.1% | Escherichia coli | |
| Vancomycin | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight stimulation | Escherichia coli | |
| additional information | no divalent cation requirement | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli JA200/pLC19-19 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 isozymes | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, in concentrated PEG 6000 solution, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
| [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli JA200/pLC19-19 | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | about 60% of maximal activity at 20°C and about half-maximal activity at 45°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
up to 87% loss of activity within 10 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | broad, in the presence of 0.1% deoxycholate | Escherichia coli |
| 8.5 | - |
Tris-HCl buffer without deoxycholate | Escherichia coli |
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