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Literature summary for 2.4.99.25 extracted from
- Cloning and characterization of the Escherichia coli heptosyltransferase III Exploring substrate specificity in lipopolysaccharide core biosynthesis (2015), FEBS Lett., 589, 1423-1429 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 0.001 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-amino-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-amino-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 0.0024 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli | |
| 0.006 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli |  |
| 0.01 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-L-glycero-beta-D-manno-heptose + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | Escherichia coli | i.e. alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-(4-O-phospho)-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A | ADP + alpha-L-glycero-D-manno-heptosyl-(1->7)-alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P25742 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-L-glycero-beta-D-manno-heptose + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-amino-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-amino-1-O-phosphono-alpha-D-glucopyranose | i.e. O-deacylated alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-(4-O-phospho)-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A | Escherichia coli | ADP + alpha-L-glycero-D-manno-heptosyl-(1->7)-alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-amino-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-amino-1-O-phosphono-alpha-D-glucopyranose | - |
ir | |
| ADP-L-glycero-beta-D-manno-heptose + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | i.e. O-deacylated alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-(4-O-phospho)-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A | Escherichia coli | ADP + alpha-L-glycero-D-manno-heptosyl-(1->7)-alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | - |
ir | |
| ADP-L-glycero-beta-D-manno-heptose + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | i.e. alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-(4-O-phospho)-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A | Escherichia coli | ADP + alpha-L-glycero-D-manno-heptosyl-(1->7)-alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HepIII | - |
Escherichia coli |
| rfaQ | - |
Escherichia coli |
| WaaQ | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0049 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli | |
| 0.019 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli | |
| 0.028 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 0.041 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 0.07 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-amino-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-amino-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 4.6 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 6.7 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-amino-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-amino-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5 | Escherichia coli | |
| 7.9 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(hydroxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli | |
| 8.3 | - |
alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100 | Escherichia coli | |
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