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Literature summary for 2.4.99.20 extracted from

  • Bacher, I.; Zidar, A.; Kratzel, M.; Hohenegger, M.
    Channelling of substrate promiscuity of the skeletal-muscle ADP-ribosyl cyclase isoform (2004), Biochem. J., 381, 147-154.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.99.20

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Oryctolagus cuniculus
additional information the cyclization reaction is insensitive to free Ca2+ concentration in the range 20 nM–100 microM. Mg2+ is not inhibitory Oryctolagus cuniculus
NAD+
-
 Oryctolagus cuniculus
NADP+
-
 Oryctolagus cuniculus
nicotinamide inhibition of the cyclization reaction and is 30fold more potent at suppressing the base-exchange reaction. Inhibition is not via a competition with the nicotinic acid-binding site Oryctolagus cuniculus
Zn2+
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0334
-
 NGDP+ pH 7.4, temperature not specified in the publication Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane of heavy sarcoplasmic reticulum Oryctolagus cuniculus 16020
-
sarcoplasmic reticulum membrane of heavy sarcoplasmic reticulum Oryctolagus cuniculus 16529
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 skeletal muscle isoform
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-phospho-cyclic ADP-ribose + nicotinate
-
 Oryctolagus cuniculus nicotinate-adenine dinucleotide phosphate
-
 ?
additional information the cyclization reaction to form cADPr and cGDPr as well as the base-exchange reaction to form nicotinic acid adenine dinucleotide phosphate are strictly dependent on pH. Although the formation of cyclic GDP-ribose is optimized at pH 6, the synthesis of nicotinic acid adenine dinucleotide phosphate is most pronounced at a pH below 5. Nicotinic acid has virtually no influence on the cyclization reaction, but increases the affinity of NADP at an acidic pH and has the opposite effect at alkaline pH Oryctolagus cuniculus ?
-
 ?
NADP+
-
 Oryctolagus cuniculus 2'-phospho-cyclic ADP-ribose + nicotinamide
-
 ?
NGDP+
-
 Oryctolagus cuniculus 2'-phospho-cyclic GDP-ribose + nicotinamide
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00027
-
 formation of cGDPr, pH 7.4, temperature not specified in the publication Oryctolagus cuniculus Cu2+
0.0013
-
 formation of cGDPr, pH 7.4, temperature not specified in the publication Oryctolagus cuniculus NADP+
0.0023
-
 formation of cGDPr, pH 7.4, temperature not specified in the publication Oryctolagus cuniculus Zn2+
0.0054
-
 formation of cGDPr, pH 7.4, temperature not specified in the publication Oryctolagus cuniculus NAD+
0.7
-
 cyclization reaction, pH 7.4, temperature not specified in the publication Oryctolagus cuniculus nicotinamide