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Literature summary for 2.4.99.18 extracted from

  • Larkin, A.; Chang, M.M.; Whitworth, G.E.; Imperiali, B.
    Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis (2013), Nat. Chem. Biol., 9, 367-373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Methanococcus voltae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme requires the addition of divalent metal cations such as Mn2+ Methanococcus voltae

Organism

Organism UniProt Comment Textmining
Methanococcus voltae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Methanococcus voltae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl monophosphate GlcNAc-Glc-2,3-diNAcA + [protein]-L-asparagine
-
Methanococcus voltae ?
-
?
additional information minimal activity with alpha-linked dolichyl monophosphate N-acetylglucosamine and no glycosylation activity with peptide Ac(YKYQESSYKpNF)NH2 Methanococcus voltae ?
-
?

Synonyms

Synonyms Comment Organism
AglB
-
Methanococcus voltae
OTase
-
Methanococcus voltae