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Literature summary for 2.4.99.18 extracted from

  • Faridmoayer, A.; Fentabil, M.A.; Haurat, M.F.; Yi, W.; Woodward, R.; Wang, P.G.; Feldman, M.F.
    Extreme substrate promiscuity of the Neisseria oligosaccharyl transferase involved in protein O-glycosylation (2008), J. Biol. Chem., 283, 34596-34604.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology promising candidate for glycoengineering, the development of glycan-based vaccines and therapeutics Neisseria meningitidis

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification, expression of histidine-tagged enzyme in Escherichia coli and Salmonella enterica strains Neisseria meningitidis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Campylobacter jejuni heptasaccharide bound to undecaprenyl diphosphate + pilin Neisseria meningitidis alpha-1,3-linked sugar, in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli group 1 K30 capsule antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O16 antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis alpha-1,6-linked Glc with GlcNAc, in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O2 antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis rhamnose beta-1,4-linked sugar, in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O7 antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis beta-1,3-linked sugar, in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O86 antigen bound to farnesyl diphosphate + pilin Neisseria meningitidis pentasaccharide attached to other lipid carrier, in vitro, 500 mM Tris-HCl containing 1 M sucrose and 10 mM MnCl2, pH7.5, 30°C farnesyl diphosphate + glycosylated pilin
-
?
peptidoglycan bound to undecaprenyl diphosphate + pilin Neisseria meningitidis transfer of subunit of the peptidoglycan petapeptide, no transfer of the complete peptidoglycan is observed, in vivo in Salmonella enterica undecaprenyl diphosphate + glycosylated pilin
-
?
Pseudomonas aeruginosa O11 antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis FucNac, in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Salmonella enterica O antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis in vivo in Escherichia coli undecaprenyl diphosphate + glycosylated pilin
-
?
Salmonella typhimurium LT2 antigen bound to undecaprenyl diphosphate + pilin Neisseria meningitidis in vivo in Escherichia coli, in vivo in Salmonella enterica serovar Typhimurium LT2 strain undecaprenyl diphosphate + glycosylated pilin
-
?

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
pathogenic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
cells centrifuged, resuspended, and washed in 20 mM phosphate buffer, pH 7.2, with 0.3 M Na Cl, cells disrupted and centrifuged, membranes separated by ultracentrifugation of supernatant, resuspended in buffer with 2% Elugent and 25 mM imidazole buffer, proteins solubilized by tumbling, centrifuged, supernatant removed, Elugenat reduced to 1% or 0.5% n-dodecyl-beta-D-maltopyranoside, loaded onto Ni-nitrilotriacetic acid agarose column, elution with buffer containing 250 mM imidazole Neisseria meningitidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Campylobacter jejuni heptasaccharide bound to undecaprenyl diphosphate + pilin alpha-1,3-linked sugar, in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli group 1 K30 capsule antigen bound to undecaprenyl diphosphate + pilin in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O16 antigen bound to undecaprenyl diphosphate + pilin alpha-1,6-linked Glc with GlcNAc, in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O2 antigen bound to undecaprenyl diphosphate + pilin rhamnose beta-1,4-linked sugar, in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O7 antigen bound to undecaprenyl diphosphate + pilin beta-1,3-linked sugar, in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Escherichia coli O86 antigen bound to farnesyl diphosphate + pilin pentasaccharide attached to other lipid carrier, in vitro, 500 mM Tris-HCl containing 1 M sucrose and 10 mM MnCl2, pH7.5, 30°C Neisseria meningitidis farnesyl diphosphate + glycosylated pilin
-
?
peptidoglycan bound to undecaprenyl diphosphate + pilin transfer of subunit of the peptidoglycan petapeptide, no transfer of the complete peptidoglycan is observed, in vivo in Salmonella enterica Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Pseudomonas aeruginosa O11 antigen bound to undecaprenyl diphosphate + pilin FucNac, in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Salmonella enterica O antigen bound to undecaprenyl diphosphate + pilin in vivo in Escherichia coli Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?
Salmonella typhimurium LT2 antigen bound to undecaprenyl diphosphate + pilin in vivo in Escherichia coli, in vivo in Salmonella enterica serovar Typhimurium LT2 strain Neisseria meningitidis undecaprenyl diphosphate + glycosylated pilin
-
?

Synonyms

Synonyms Comment Organism
oligosaccharyltransferase
-
Neisseria meningitidis
OTase
-
Neisseria meningitidis
PglL
-
Neisseria meningitidis

General Information

General Information Comment Organism
physiological function O-glycosylation of type IV pilins in bacteria Neisseria meningitidis