Application | Comment | Organism |
---|---|---|
biotechnology | promising candidate for glycoengineering, the development of glycan-based vaccines and therapeutics | Neisseria meningitidis |
Cloned (Comment) | Organism |
---|---|
PCR-amplification, expression of histidine-tagged enzyme in Escherichia coli and Salmonella enterica strains | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Campylobacter jejuni heptasaccharide bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | alpha-1,3-linked sugar, in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli group 1 K30 capsule antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O16 antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | alpha-1,6-linked Glc with GlcNAc, in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O2 antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | rhamnose beta-1,4-linked sugar, in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O7 antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | beta-1,3-linked sugar, in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O86 antigen bound to farnesyl diphosphate + pilin | Neisseria meningitidis | pentasaccharide attached to other lipid carrier, in vitro, 500 mM Tris-HCl containing 1 M sucrose and 10 mM MnCl2, pH7.5, 30°C | farnesyl diphosphate + glycosylated pilin | - |
? | |
peptidoglycan bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | transfer of subunit of the peptidoglycan petapeptide, no transfer of the complete peptidoglycan is observed, in vivo in Salmonella enterica | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Pseudomonas aeruginosa O11 antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | FucNac, in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Salmonella enterica O antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | in vivo in Escherichia coli | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Salmonella typhimurium LT2 antigen bound to undecaprenyl diphosphate + pilin | Neisseria meningitidis | in vivo in Escherichia coli, in vivo in Salmonella enterica serovar Typhimurium LT2 strain | undecaprenyl diphosphate + glycosylated pilin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
pathogenic bacterium | - |
Purification (Comment) | Organism |
---|---|
cells centrifuged, resuspended, and washed in 20 mM phosphate buffer, pH 7.2, with 0.3 M Na Cl, cells disrupted and centrifuged, membranes separated by ultracentrifugation of supernatant, resuspended in buffer with 2% Elugent and 25 mM imidazole buffer, proteins solubilized by tumbling, centrifuged, supernatant removed, Elugenat reduced to 1% or 0.5% n-dodecyl-beta-D-maltopyranoside, loaded onto Ni-nitrilotriacetic acid agarose column, elution with buffer containing 250 mM imidazole | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Campylobacter jejuni heptasaccharide bound to undecaprenyl diphosphate + pilin | alpha-1,3-linked sugar, in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli group 1 K30 capsule antigen bound to undecaprenyl diphosphate + pilin | in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O16 antigen bound to undecaprenyl diphosphate + pilin | alpha-1,6-linked Glc with GlcNAc, in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O2 antigen bound to undecaprenyl diphosphate + pilin | rhamnose beta-1,4-linked sugar, in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O7 antigen bound to undecaprenyl diphosphate + pilin | beta-1,3-linked sugar, in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Escherichia coli O86 antigen bound to farnesyl diphosphate + pilin | pentasaccharide attached to other lipid carrier, in vitro, 500 mM Tris-HCl containing 1 M sucrose and 10 mM MnCl2, pH7.5, 30°C | Neisseria meningitidis | farnesyl diphosphate + glycosylated pilin | - |
? | |
peptidoglycan bound to undecaprenyl diphosphate + pilin | transfer of subunit of the peptidoglycan petapeptide, no transfer of the complete peptidoglycan is observed, in vivo in Salmonella enterica | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Pseudomonas aeruginosa O11 antigen bound to undecaprenyl diphosphate + pilin | FucNac, in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Salmonella enterica O antigen bound to undecaprenyl diphosphate + pilin | in vivo in Escherichia coli | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? | |
Salmonella typhimurium LT2 antigen bound to undecaprenyl diphosphate + pilin | in vivo in Escherichia coli, in vivo in Salmonella enterica serovar Typhimurium LT2 strain | Neisseria meningitidis | undecaprenyl diphosphate + glycosylated pilin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oligosaccharyltransferase | - |
Neisseria meningitidis |
OTase | - |
Neisseria meningitidis |
PglL | - |
Neisseria meningitidis |
General Information | Comment | Organism |
---|---|---|
physiological function | O-glycosylation of type IV pilins in bacteria | Neisseria meningitidis |