Literature summary for 2.4.99.18 extracted from

Li, L.; Woodward, R.; Ding, Y.; Liu, X.W.; Yi, W.; Bhatt, V.S.; Chen, M.; Zhang, L.W.; Wang, P.G.
Overexpression and topology of bacterial oligosaccharyltransferase PglB (2010), Biochem. Biophys. Res. Commun., 394, 1069-1074.

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Cloned(Commentary)

Cloned (Comment)	Organism
PglB is overexpressed in Escherichia coli C43(DE3) at a level of 1 mg/L cell cultures	Campylobacter jejuni

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain	Campylobacter jejuni	16020	-

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	Q9S4V7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pentaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine	PglB can utilize shorter polyisoprenol (pentaprenol) as the lipid carrier, albeit with reduced efficiency	Campylobacter jejuni	pentaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine	-	?
undecaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine	PglB is solely responsible for the oligosaccharyltransferase activity. PglB can transfer a monosaccharide, e.g. GalNAc to a peptide acceptor. PglB exhibits relaxed sugar substrate specificity	Campylobacter jejuni	undecaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine	-	?

Synonyms

Synonyms	Comment	Organism
PglB	-	Campylobacter jejuni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Campylobacter jejuni

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Release 2023.1 (January 2023)