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Literature summary for 2.4.99.18 extracted from
- Oligosaccharyltransferase activity is associated with a protein complex composed of ribophorins I and II and a 48 kd protein (1992), Cell, 69, 55-65.
General Stability
| General Stability | Organism |
|---|---|
| phosphatidylcholine stabilizes detergent-solubilized enzyme | Canis lupus familiaris |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| rough endoplasmic reticulum | ribophorin I and II are integral membrane glycoproteins | Canis lupus familiaris | 5791 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 66000 + x * 63000 + x * 48000, SDS-PAGE | Canis lupus familiaris |
| 63000 | - |
x * 66000 + x * 63000 + x * 48000, SDS-PAGE | Canis lupus familiaris |
| 66000 | - |
x * 66000 + x * 63000 + x * 48000, SDS-PAGE | Canis lupus familiaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Canis lupus familiaris | central enzyme in synthesis of N-linked glycoproteins | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000985 | - |
- |
Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Canis lupus familiaris | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
| dolichyl diphosphooligosaccharide + synthetic tripeptides | Asn-Tyr-Thr | Canis lupus familiaris | ? | - |
? | |
| lipid-linked oligosaccharide + unfolded nascent polypeptide chain | central enzyme in synthesis of N-linked glycoproteins | Canis lupus familiaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000 + x * 63000 + x * 48000, SDS-PAGE | Canis lupus familiaris |
| More | oligosaccharyltransferase activity is mediated by a protein complex composed of ribophorin I, 66000 Da, and ribophorin II, 63000 Da, and a 48000 Da protein | Canis lupus familiaris |
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Release 2023.1 (January 2023)
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