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Literature summary for 2.4.3.9 extracted from

  • Prinetti, A.; Aureli, M.; Illuzzi, G.; Prioni, S.; Nocco, V.; Scandroglio, F.; Gagliano, N.; Tredici, G.; Rodriguez-Menendez, V.; Chigorno, V.; Sonnino, S.
    GM3 synthase overexpression results in reduced cell motility and in caveolin-1 upregulation in human ovarian carcinoma cells (2010), Glycobiology, 20, 62-77.
    View publication on PubMed
Search for more literature for 2.4.3.9

Cloned(Commentary)

Cloned (Comment) Organism
SAT-1 cDNA cloned into plasmid expression vector pRc/CMV under control of CMV promoter, overexpression in A2780 ovarian tumor cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information motility of low GM3 synthase expressing cells is reduced in the presence of a Src kinase inhibitor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-Neu5Ac + lactosylceramide caveolin-1 is a GM3-interacting protein in GM3 synthase overexpressing cells Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GM3 synthase
-
 Homo sapiens
SAT-1
-
 Homo sapiens
sialyltransferase-1
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens SAT-1 mRNA level and GM3 synthase activity in vitro are markedly upregulated in 3 SAT-1-transfected clones with respect to wild-type and mock-transfected A2780 cells up

General Information

General Information Comment Organism
physiological function overexpression of GM3 synthase in A2780 cells consistently results in elevated ganglioside (GM3, GM2 and GD1a) levels. GM3 synthase overexpressing cells have a growth rate similar to wild-type cells, but show a strongly reduced in vitro cell motility accompanied by reduced levels of the epithelial-mesenchymal transition marker alpha smooth muscle actin. Caveolin-1 is markedly upregulated in GM3 synthase overexpressing cells. Higher levels of the inactive form of c-Src are present in GM3 synthase overexpressing cells, associated with a ganglioside- and caveolin-rich detergent insoluble fraction Homo sapiens