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Literature summary for 2.4.3.9 extracted from
- Characterization of a CMP-sialic acid:lactosylceramide sialyltransferase activity in cultured hamster cells (1984), Biochim. Biophys. Acta, 804, 442-449.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cutscum | - |
Mesocricetus auratus | |
| additional information | not activated by Tween 20 or 80 | Mesocricetus auratus | |
| Triton CF-54 | activation, most potent activator | Mesocricetus auratus |  |
| Triton CF-54/Tween 80 | - |
Mesocricetus auratus | |
| Triton X-100 | activation | Mesocricetus auratus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| asialofetuin | weak | Mesocricetus auratus | |
| ganglioside GM3 | 0.5 mM and 1 mM, 43% and 60% inhibition respectively | Mesocricetus auratus | |
| Globotetraosylceramide | weak | Mesocricetus auratus | |
| ionic detergents | - |
Mesocricetus auratus | |
| additional information | glucosylceramide or globotriaosylceramide | Mesocricetus auratus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
lactosylceramide | - |
Mesocricetus auratus | |
| 0.16 | - |
CMP-N-acetylneuraminate | - |
Mesocricetus auratus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, slightly less efficient than Mn2+ | Mesocricetus auratus | |
| Mg2+ | activation, slightly less efficient than Mn2+ | Mesocricetus auratus | |
| Mn2+ | 15 mM, required for maximal activity | Mesocricetus auratus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + lactosylceramide | Mesocricetus auratus | involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids | CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Mesocricetus auratus | - |
| fibroblast | contact-inhibited cell-line NIL-8 | Mesocricetus auratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
Mesocricetus auratus | CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | i.e. ganglioside GM3 | ? | |
| CMP-N-acetylneuraminate + lactosylceramide | involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids | Mesocricetus auratus | CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
no effects of buffer on activity | Mesocricetus auratus |
| 6.5 | - |
broad optimum in cacodylate-HCl buffer | Mesocricetus auratus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | - |
Mesocricetus auratus |
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