Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, N-terminally truncated form | Vibrio sp. JT-FAJ-16 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | in presence of 500-700 mM NaCl 2fold increase in activity | Vibrio sp. JT-FAJ-16 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43921 | - |
MALDI-TOF of truncated enzyme with N-terminal Lys2 to Cys22 deletion | Vibrio sp. JT-FAJ-16 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. JT-FAJ-16 | A8R0Y0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated enzyme | Vibrio sp. JT-FAJ-16 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-Glc | - |
Vibrio sp. JT-FAJ-16 | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-beta-D-GlcNAc | 127% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylgalactosamine | 58% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + alpha-Neu5Ac-(2->3)-beta-D-GalNAc | - |
? | |
CMP-N-acetyl-beta-neuraminate + beta-D-N-acetylglucosamine | 55% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + alpha-Neu5Ac-(2->3)-beta-D-GlcNAc | - |
? | |
CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->3)-beta-D-GlcNAc | 124% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc | - |
? | |
CMP-N-acetyl-beta-neuraminate + methyl beta-D-galactopyranoside | 114% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-galactopyranoside | - |
? | |
CMP-N-acetyl-beta-neuraminate + methyl beta-D-glucopyranoside | 52% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-glucopyranoside | - |
? | |
CMP-N-acetyl-beta-neuraminate + methyl beta-D-mannopyranoside | 57% of the activity with beta-D-Gal-(1->4)-beta-D-Glc, recombinant enzyme | Vibrio sp. JT-FAJ-16 | CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Man | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43921, MALDI-TOF of truncated enzyme with N-terminal Lys2 to Cys22 deletion | Vibrio sp. JT-FAJ-16 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Vibrio sp. JT-FAJ-16 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | 10°C: 65% of maximal activity, 30°C: 80% of maximal activity | Vibrio sp. JT-FAJ-16 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Vibrio sp. JT-FAJ-16 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5: about 40% of maximal activity, pH 9: about 60% of maximal actzivity | Vibrio sp. JT-FAJ-16 |